Phenotypes associated with this allele

• Allele Symbol: Rr695571^tm1Dlsi
• Allele Name: targeted mutation 1, Debra L Silver
• Allele ID: MGI:8237986
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rr695571^tm1Dlsi/Rr695571^tm1Dlsi	C57BL/6J-Rr695571^tm1Dlsi	MGI:8238038
cn2	Emx1^tm1(cre)Krj/Emx1^tm1(cre)Krj Rr695571^tm1Dlsi/Rr695571^tm1Dlsi	involves: 129S2/SvPas * C57BL/6J	MGI:8238041

Genotype
MGI:8238038

hm1

• Allelic Composition: Rr695571^tm1Dlsi/Rr695571^tm1Dlsi
• Genetic Background: C57BL/6J-Rr695571^tm1Dlsi

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal radial glial cell morphology ( J:366779 )

• increased fraction of mitotic RGCs in E12.5 embryos

• reduced cell-cycle exit of RGC progenitors between stage E11.5 and E12.5 embryos

• increased differentiation into intermediate progenitors (IPs) and newborn neurons in cerebral cortex of E15.5 embryos

increased radial glial cell number ( J:366779 )

• 11% more SOX2+ RGCs in cerebral cortex in E14.5 embryos

• 28% increase in intermediate progenitors (IPs) in cerebral cortex of E14.5 embryos

• 33% increase in TBR2+ SOX2+ intermediate progenitors (IPS) in cerebral cortex of E14.5 embryos

nervous system

nervous system phenotype ( J:366779 )

N • normal global synapsin1+ number in adult somatosensory cortex (S1bf)

N • normal oligodendrocyte and astrocyte number in adult cerebral cortex

abnormal radial glial cell morphology ( J:366779 )

• increased fraction of mitotic RGCs in E12.5 embryos

• reduced cell-cycle exit of RGC progenitors between stage E11.5 and E12.5 embryos

• increased differentiation into intermediate progenitors (IPs) and newborn neurons in cerebral cortex of E15.5 embryos

increased radial glial cell number ( J:366779 )

• 11% more SOX2+ RGCs in cerebral cortex in E14.5 embryos

• 28% increase in intermediate progenitors (IPs) in cerebral cortex of E14.5 embryos

• 33% increase in TBR2+ SOX2+ intermediate progenitors (IPS) in cerebral cortex of E14.5 embryos

abnormal neocortex morphology ( J:366779 )

• higher ratio of upper layers (II, III, IV) versus deep layers (V, VI) at age P21

increased neocortex size ( J:366779 )

• increased area at age 3 months

• more NeuN+ excitatory neurons at age P21

abnormal nervous system physiology ( J:366779 )

• more functionally independent activity between cortical regions in primary sensory (whisker barrel field, S1bf), premotor (rostral and caudal M2) and higher-order associative regions (retrosplenial, RSP)

Genotype
MGI:8238041

cn2

• Allelic Composition: Emx1^tm1(cre)Krj/Emx1^tm1(cre)Krj; Rr695571^tm1Dlsi/Rr695571^tm1Dlsi
• Genetic Background: involves: 129S2/SvPas * C57BL/6J

cellular

abnormal radial glial cell morphology ( J:366779 )

• increased cell-cycle exit of RGC progenitors between stage E11.5 and E12.5 embryos

• normal fraction of mitotic RGCs in E12.5 embryos

nervous system

abnormal radial glial cell morphology ( J:366779 )

• increased cell-cycle exit of RGC progenitors between stage E11.5 and E12.5 embryos

• normal fraction of mitotic RGCs in E12.5 embryos

decreased neocortex size ( J:366779 )

• at age 3 months