Phenotypes associated with this allele

• Allele Symbol: Ddit4l^em1Omunc
• Allele Name: endonuclease-mediated mutation 1, Naoki Harada
• Allele ID: MGI:8224528
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ddit4l^em1Omunc/Ddit4l^em1Omunc	C57BL/6J-Ddit4l^em1Omunc	MGI:8226101

Genotype
MGI:8226101

hm1

• Allelic Composition: Ddit4l^em1Omunc/Ddit4l^em1Omunc
• Genetic Background: C57BL/6J-Ddit4l^em1Omunc

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:370104 )

♂N • male mice show normal hepatic triglyceride and cholesterol levels, regardless of diet

increased insulin secretion ( J:370104 )

♂ • male mice fed a high-fat diet (HFD) show increased plasma insulin levels at 15 min after intraperitoneal glucose injection

improved glucose tolerance ( J:370104 )

♂ • in an intraperitoneal glucose tolerance test (IPGTT), 16-week-old male mice fed a HFD show improved glucose tolerance, with a significantly decreased area under the curve (AUC) relative to HFD-fed wild-type males

♂ • however, males fed a standard diet (STD) show no differences in glucose tolerance relative to STD-fed wild-type males

♂ • moreover, 17-week-old males show normal insulin sensitivity whether fed a STD or HFD

endocrine/exocrine glands

decreased pancreatic beta cell apoptosis ( J:370104 )

♂ • HFD-fed males show a decrease in the number of TUNEL+ apoptotic pancreatic beta cells relative to HFD-fed wild-type males

♂ • however, the proportion of Ki67+ proliferative beta cells is unchanged after HFD feeding

increased pancreatic beta cell mass ( J:370104 )

♂ • HFD-fed males show a significant increase in pancreatic beta-cell area and the beta cell mass to body weight ratio relative to HFD-fed wild-type males

increased insulin secretion ( J:370104 )

♂ • male mice fed a high-fat diet (HFD) show increased plasma insulin levels at 15 min after intraperitoneal glucose injection

adipose tissue

decreased inguinal fat pad weight ( J:370104 )

♂ • STD-fed male mice show a slight but statistically significant reduction in inguinal subcutaneous white adipose tissue (WAT) relative to STD-fed wild-type males, with no detectable differences after HFD feeding

♂ • both STD- and HFD-fed males show no significant changes in the relative weight of hamstring and gastrocnemius muscles, adipose tissues (mesenteric WAT, perirenal WAT, epididymal WAT, and subscapular WAT and BAT), or various organs relative diet-matched wild-type controls

cellular

decreased pancreatic beta cell apoptosis ( J:370104 )

♂ • HFD-fed males show a decrease in the number of TUNEL+ apoptotic pancreatic beta cells relative to HFD-fed wild-type males

♂ • however, the proportion of Ki67+ proliferative beta cells is unchanged after HFD feeding

growth/size/body

growth/size/body region phenotype ( J:370104 )

♂N • male mice fed a standard diet or HFD show no significant changes in body weight gain relative to diet-matched wild-type controls

liver/biliary system

liver/biliary system phenotype ( J:370104 )

♂N • male mice show normal liver weight relative to body weight, regardless of diet