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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Zmym2Tn(pb-Act-RFP)1Xwu
transposon insertion 1, Xiaohui Wu
MGI:8215475
Summary 2 genotypes


Genotype
MGI:8217231
hm1
Allelic
Composition
Zmym2Tn(pb-Act-RFP)1Xwu/Zmym2Tn(pb-Act-RFP)1Xwu
Genetic
Background
FVB/N-Zmym2Tn(pb-Act-RFP)1Xwu
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Zmym2Tn(pb-Act-RFP)1Xwu mutation (0 available); any Zmym2 mutation (76 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• although present at Mendelian ratios until E9.5, all homozygous embryos are resorbed by E10.5; no live homozygous mice are recovered from heterozygous intercrosses

embryo
• embryos exhibit severe growth retardation by E9.5
• at E9.5, but not at E8.5, most embryos exhibit a significant reduction in crown to tail length relative to wild-type controls

growth/size/body
• embryos exhibit severe growth retardation by E9.5
• at E9.5, but not at E8.5, most embryos exhibit a significant reduction in crown to tail length relative to wild-type controls




Genotype
MGI:8217232
ht2
Allelic
Composition
Zmym2Tn(pb-Act-RFP)1Xwu/Zmym2+
Genetic
Background
FVB/N-Zmym2Tn(pb-Act-RFP)1Xwu
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Zmym2Tn(pb-Act-RFP)1Xwu mutation (0 available); any Zmym2 mutation (76 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• a small proportion of heterozygous mice die after P60, with 10.7% dying by P180
• however, no significant deterioration in kidney function is noted at the time of death

renal/urinary system
• at E12.5, kidney primordia show a significant increase in the number of Caspase3-positive apoptotic cells in the ureteric bud (UB) and nephric duct regions
• 28 of 60 newborn mice exhibit congenital anomalies of the kidney and urinary tract (CAKUT) phenotypes, not detectable in wild-type controls
• however, no significant differences in phenotype distribution are noted between sexes or between left and right kidneys
• newborns with unilateral hydronephrosis show a reduction in the number of renal glomeruli
• however, newborns with unilateral duplex kidneys show normal morphology of renal glomeruli
• furthermore, H&E staining of kidney sections shows no structural anomalies in the glomeruli at 8 and 12 weeks of age
• mice show a ~50% reduction in TBX18 (T-box18) protein expression in kidney tissues
• kidneys exhibit discontinuous nephrogenic zones, with abnormal extension of the nephrogenic zone deep into the kidney contributing to the formation of duplex kidneys
• 13 of 60 (21.7%) newborns exhibit a unilateral duplex kidney, as defined by the presence of double renal pelvises
• 9 of 60 (15%) newborns exhibit unilateral hydronephrosis
• newborns with unilateral hydronephrosis show a reduction in the number of kidney tubules
• however, newborns with unilateral duplex kidneys show normal morphology of kidney tubules
• 13 of 60 (21.7%) newborns exhibit a unilateral duplex kidney, as defined by the presence of double renal pelvises
• 6 of 60 (10%) newborns exhibit unilateral renal agenesis
• at E16.5, unilateral duplicated kidneys are accompanied with double ureters
• at E12.5, nine of 36 (25.0%) kidney primordia exhibit two ureteric buds (UBs) formed from one nephric duct, indicating duplicated UB formation during early metanephric development
• 13 of the 28 (46.4%) newborn mice with CAKUT phenotypes show unilateral vesicoureteral reflux (VUR); these include 4 cases from the unilateral duplex kidney group, 6 cases from the unilateral hydronephrosis group, and 3 cases from the single kidney group

reproductive system
N
• adult mice exhibit normal fertility; despite abnormalities in testis, seminal vesicle, and cauda epididymis morphology and impaired sperm motility, mature epididymal sperm appear normal with typical sperm head morphology, total epididymal sperm count is unchanged, and male fertility (vaginal plug formation, pregnancy rate, and litter size) is unaffected when wild-type females from different strains (129S and C57BL/6) are used
• adult males show a significant reduction in average path velocity (VAP), straight-line velocity (vSL) and curvilinear velocity (vCL); the % of progressive mobile sperm (level a and b) is slightly lower, whereas the % of sperm with slow velocity (level c) is significantly higher than in wild-type controls
• adult males with seminal vesicle atrophy show a 32% reduction in seminal vesicle weight relative to wild-type males
• 7 of 9 (77.78%) adult males show seminal vesicle atrophy, with the mucosal folds of the seminal vesicles found to be missing or reduced
• 1 of 9 (11.11%) adult males exhibit intratesticular cysts
• 1 of 9 (11.11%) adult males exhibit bilateral cryptorchidism with hydrocele
• 2 of 9 (22.22%) adult males exhibit unilateral cryptorchidism
• 1 of 9 (11.11%) adult males exhibit bilateral cryptorchidism with hydrocele

endocrine/exocrine glands
• adult males with seminal vesicle atrophy show a 32% reduction in seminal vesicle weight relative to wild-type males
• 7 of 9 (77.78%) adult males show seminal vesicle atrophy, with the mucosal folds of the seminal vesicles found to be missing or reduced
• 1 of 9 (11.11%) adult males exhibit intratesticular cysts
• 1 of 9 (11.11%) adult males exhibit bilateral cryptorchidism with hydrocele
• 2 of 9 (22.22%) adult males exhibit unilateral cryptorchidism
• 1 of 9 (11.11%) adult males exhibit bilateral cryptorchidism with hydrocele
• during an oral glucose tolerance test, 90-day-old mice exhibit a lower area under the curve (AUC) for insulin at 30 min, but not at 60 or 90 min, post-glucose administration, indicating reduced insulin secretory capacity
• however, no changes in pancreatic morphology or histology are observed

behavior/neurological
N
• mice exhibit normal dietary habits and activity levels relative to wild-type controls
• in the resident-intruder paradigm, resident male mice show no major changes in the latency to the first biting attack or the frequency of biting attacks during the first and second trials; however, in the third trial, the frequency of biting attacks is significantly higher than that in wild-type males, indicating increased inter-male aggression after repeated encounters with intruders
• however, walking duration and the number of rearings during the first trial are not affected
• in an open-field test, adult male mice are significantly more active during the first 15 min of the test and spend less time in the center zone than wild-type males, suggesting increased anxiety-like behavior
• however, adult female mice show normal anxiety levels

cellular
• at E12.5, kidney primordia show a significant increase in the number of Caspase3-positive apoptotic cells in the ureteric bud (UB) and nephric duct regions
• adult males show a significant reduction in average path velocity (VAP), straight-line velocity (vSL) and curvilinear velocity (vCL); the % of progressive mobile sperm (level a and b) is slightly lower, whereas the % of sperm with slow velocity (level c) is significantly higher than in wild-type controls

homeostasis/metabolism
N
• mice exhibit normal blood urea nitrogen (BUN) and serum creatinine levels over a 180-day observation period, indicating normal kidney function
• during an oral glucose tolerance test, 90-day-old mice exhibit a lower area under the curve (AUC) for insulin at 30 min, but not at 60 or 90 min, post-glucose administration, indicating reduced insulin secretory capacity
• however, no changes in pancreatic morphology or histology are observed
• at P90, both random and fasting blood glucose levels are significantly elevated and remain stable throughout the study
• mice develop chronic but mild hyperglycemia, with blood glucose levels progressively increasing until 90 days of age
• mice exhibit significantly reduced fasting serum insulin concentrations

growth/size/body
N
• mice exhibit normal body weight from P0 to P120 relative to wild-type controls
• 1 of 9 (11.11%) adult males exhibit intratesticular cysts

nervous system
N
• adult mice show no apparent defects in gross brain structure and histopathology (H&E staining) of brain tissue

craniofacial
N
• adult mice exhibit no obvious craniofacial defects

integument
N
• mice exhibit normal fur appearance over an observation period of 12 weeks





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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory