Analysis Tools
integument
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• mice subcutaneously injected with pruritic compounds into the nape show a strong reduction in acute scratching behavior upon histamine application compared to controls
• mice injected with chloroquine (MrgprA3 activator) and BAM8-22 (MrgprC11 activator) show a robust decrease in bouts of scratching
• however, residual scratching that is delayed in response to chloroquine is seen which may be due to off-target effects of chloroquine
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nervous system
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• DRGs treated with a brief chloroquine application and washout exhibit a depolarized shift in the resting membrane potential
• however, action potential amplitudes and the thresholds to elicit action potentials are not affected
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• action potentials kinetics in DRGs are affected, showing a higher time to reach the maximum peak and a lower time to reach the minimum value in the repolarization phase
• DRGs are not affected by the addition of chloroquine compared to wild-type DRGs which show a lower amplitude and a higher action potential threshold in response to chloroquine
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• analysis of calcium signals after the application of pruritogens in isolated dorsal root ganglia (DRGs) shows a reduction in the total number of DRG neurons that respond to histamine and to chloroquine
• however, peak calcium responses to pruritogens in DRGs are like those in controls
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