Phenotypes associated with this allele

• Allele Symbol: Paupar^tm1.1Suss
• Allele Name: targeted mutation 1.1, Lori Sussel
• Allele ID: MGI:8213455
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Paupar^tm1.1Suss/Paupar^tm1.1Suss	involves: 129 * C57BL/6J	MGI:8213541

Genotype
MGI:8213541

hm1

• Allelic Composition: Paupar^tm1.1Suss/Paupar^tm1.1Suss
• Genetic Background: involves: 129 * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

abnormal pancreatic alpha cell morphology ( J:282894 )

• total islet glucagon content is 71% less than in controls

decreased pancreatic alpha cell mass ( J:282894 )

• at 6 weeks of age alpha cells comprise only ~6% of islets compared to ~13% in wild-type controls at 6 weeks of age

increased pancreatic alpha cell number ( J:282894 )

• significant alpha cell and islet hyperplasia at 7 months of age

increased pancreatic beta cell mass ( J:282894 )

• modest increase in beta cell area, ~79% compared to ~71% in controls at 6 weeks of age

decreased glucagon secretion ( J:282894 )

• cultured islets secrete ~60% less glucagon compared to controls in response to low glucose and ~47% less in response to 10 mM arginine

increased glucagon secretion ( J:282894 )

• when glucagon secretion is normalized to islet glucagon content islet cells hypersecrete glucagon in high glucose conditons

homeostasis/metabolism

abnormal glucose homeostasis ( J:282894 )

• significantly hypoglycemic throughout an insulin tolerance test at 6 weeks of age compared to similarly treated wild-type mice

• however, mice over 6 months of age are hyperglycemic throughout an insulin tolerance test

decreased glucagon secretion ( J:282894 )

• cultured islets secrete ~60% less glucagon compared to controls in response to low glucose and ~47% less in response to 10 mM arginine

increased glucagon secretion ( J:282894 )

• when glucagon secretion is normalized to islet glucagon content islet cells hypersecrete glucagon in high glucose conditons

decreased fasting circulating glucose level ( J:282894 )

• become significantly hypoglycemic following an overnight fast at 6 weeks of age

• however, by 3 and 6 months of age mice are no longer hypoglycemic following an overnight fast

decreased circulating glucagon level ( J:282894 )

• 30 min after an insulin injection mice have a ~2.5 fold decrease in plasma glucagon relative to wild-type mice