Phenotypes associated with this allele

• Allele Symbol: Apol7c^em4Gpt
• Allele Name: endonuclease-mediated mutation 4, GemPharmatech
• Allele ID: MGI:8205014
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Apol7c^em4Gpt/Apol7c^em4Gpt	C57BL/6JGpt-Apol7c^em4Gpt/Gpt	MGI:8205143

Genotype
MGI:8205143

hm1

• Allelic Composition: Apol7c^em4Gpt/Apol7c^em4Gpt
• Genetic Background: C57BL/6JGpt-Apol7c^em4Gpt/Gpt

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal dendritic cell physiology ( J:362930 )

• treatment of FLT3-cDCs with polyinosinic:polycytidylic acid [poly(I:C)], does not increase the number of galectin 3+ phagosomes as is seen in wild-type FLT3L-cDCs indicating that phagosomal rupture is impaired

• however, mice exhibit normal myeloid and lymphoid composition, including similar amounts of type 1 conventional dendritic cells (cDC1s) and type 2 conventional dendritic cells (cDC2s)

abnormal dendritic cell antigen presentation ( J:362930 )

• FLT3L-cDCs have a defect in ability to cross-present OVA-beads to both B3Z cells and to OT-I cells

• FLT3L-cDC1s have a large defect in their ability to cross-present OVA-beads

• FLT3L-cDC2s have a modest defect in their ability to cross-present OVA-beads

• however, neither FLT3L-cDC1s nor FLT3L-cDC2s have any defect in their ability to directly present OVA SIINFEKL peptide

• mice immunized with OVA-beads + poly(I:C) show a reduced OVA-specific CD8+ T cell response

• mice immunized with ultraviolet-irradiated, OVA-coated NIH-3T3 cells (H-2^q) + poly(I:C) show a reduced OVA-specific CD8+ T cell response indicating an inability to cross-present dead cell-associated antigens