Phenotypes associated with this allele

• Allele Symbol: Prmt9^em1Sqiu
• Allele Name: endonuclease-mediated mutation 1, Shenfeng Qiu
• Allele ID: MGI:8166513
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Prmt9^em1Sqiu/Prmt9^em1Sqiu Emx1^tm1(cre)Krj/Emx1^+	B6.Cg-Emx1^tm1(cre)Krj Prmt9^em1Sqiu	MGI:8166762

Genotype
MGI:8166762

cn1

• Allelic Composition: Prmt9^em1Sqiu/Prmt9^em1Sqiu; Emx1^tm1(cre)Krj/Emx1⁺
• Genetic Background: B6.Cg-Emx1^tm1(cre)Krj Prmt9^em1Sqiu

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

impaired contextual conditioning behavior ( J:360860 )

• mice show less conditioned contextual freezing during the test phase of the fear conditioning test

• however, no differences in the open field or elevated plus maze are seen indicating normal basal locomotor activity and levels of anxiety or reaction to novelty

impaired cued conditioning behavior ( J:360860 )

• in the fear conditioning test, mice are slower in associating an auditory tone (conditioned stimulus) with a foot shock (unconditioned stimulus)

impaired spatial learning ( J:360860 )

• 10-14-week-old mice show slower spatial memory acquisition during the Morris water maze training phase

abnormal long-term spatial reference memory ( J:360860 )

• 10-14-week-old mice spend less time in the target quadrant during the Morris water maze probe trial

impaired spatial memory extinction ( J:360860 )

• in the reverse learning test, during which the platform is moved to a new location, mice exhibit slower learning of the new platform location, indicating impaired cognitive flexibility

nervous system

decreased CNS synapse formation ( J:360860 )

• hippocampal neurons show impaired excitatory synapse maturation

decreased dendritic spine density ( J:360860 )

• hippocampus CA1 neurons show a reduction in spine density and head volume, indicating defective synapse development

• however, dendritic arborization is not affected

abnormal excitatory synapse morphology ( J:360860 )

• hippocampus neurons show impaired excitatory synapse development

• primary embryonic hippocampus neurons show a reduced density of PSD95+, GluN1+, and GluA1+ puncta and decreased colocalization of pre-/post-synaptic and AMPA/NMDA markers, indicating reduction in the number of functional excitatory synapses

decreased excitatory postsynaptic current amplitude ( J:360860 )

• CA1 neurons from young adults (12-14 weeks old) show reduced spontaneous miniature excitatory postsynaptic currents (mEPSCs) amplitude, with more mEPSCs amplitudes clustered to the lower amplitude bins

decreased excitatory postsynaptic current frequency ( J:360860 )

• mEPSC frequency is decreased in CA1 neurons

abnormal excitatory postsynaptic potential ( J:360860 )

• overall reduction in postsynaptic fEPSP response to presynaptic inputs in CA1 responses, while paired pulse ratio responses across various inter-stimulus intervals are not altered

abnormal glutamate-mediated receptor currents ( J:360860 )

• decrease in AMPA/NMDA receptor current ratio in CA1 neurons

reduced long-term potentiation ( J:360860 )

• reduction of long-term potentiation (LTP) from CA1 region

reduced long-term depression ( J:360860 )

• reduction of long-term depression (LTD) from CA1 region