Analysis Tools
reproductive system
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• RNA surveillance - responsible for the degradation of waste RNAs in the nucleus - is disrupted, leading to the accumulation of RNAs with unprocessed introns and incomplete transcript structures in the oocyte nucleus
• non-canonical H3K4me3 (histone H3 lysine-4 trimethylation) on gene bodies is disrupted, leading to impaired genome silencing and failure to achieve normal oocyte growth
• accumulation of unprocessed RNAs in the nucleus results in the loss of chromatin structure and failure of configuration transition during oocyte growth
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• although growing oocytes derived from day-14 mice have normal size and morphology, oocytes show impaired growth from day 14 to day 21, resulting in a significantly decreased oocyte diameter at day 21
• in addition to smaller size, oocytes exhibit a thinner zona pellucida, enlarged perivitelline space, and increased granularity in the ooplasm
• oocytes from day-21 mice show large-scale accumulation of RNAs in the nucleus and blockage of cytoplasmic mRNA storage
• immunofluorescent staining of PABPN1 and SRSF2 shows absence of nuclear speckles in oocytes derived from day-21 mice
• growing oocytes from day-14 mice show an non-surrounded nucleoli (NSN) chromatin configuration, whereas all oocytes from day-21 mice fail to accomplish the NSN-to-SN (surrounded nucleoli) transition
• no nucleolus-like structures are formed in day-21 oocytes
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• during in vitro maturation, oocytes isolated from day-21 mice and primed with PMSG for 48 h fail to undergo germinal vesicle breakdown; no metaphase II eggs are obtained after the superovulation treatment
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• female mice are completely infertile
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cellular
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• immunofluorescent staining of PABPN1 and SRSF2 shows absence of nuclear speckles in oocytes derived from day-21 mice, indicating impaired RNA processing function
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• RNA surveillance - responsible for the degradation of waste RNAs in the nucleus - is disrupted, leading to the accumulation of RNAs with unprocessed introns and incomplete transcript structures in the oocyte nucleus
• non-canonical H3K4me3 (histone H3 lysine-4 trimethylation) on gene bodies is disrupted, leading to impaired genome silencing and failure to achieve normal oocyte growth
• accumulation of unprocessed RNAs in the nucleus results in the loss of chromatin structure and failure of configuration transition during oocyte growth
|
|
|
• although growing oocytes derived from day-14 mice have normal size and morphology, oocytes show impaired growth from day 14 to day 21, resulting in a significantly decreased oocyte diameter at day 21
• in addition to smaller size, oocytes exhibit a thinner zona pellucida, enlarged perivitelline space, and increased granularity in the ooplasm
• oocytes from day-21 mice show large-scale accumulation of RNAs in the nucleus and blockage of cytoplasmic mRNA storage
• immunofluorescent staining of PABPN1 and SRSF2 shows absence of nuclear speckles in oocytes derived from day-21 mice
• growing oocytes from day-14 mice show an non-surrounded nucleoli (NSN) chromatin configuration, whereas all oocytes from day-21 mice fail to accomplish the NSN-to-SN (surrounded nucleoli) transition
• no nucleolus-like structures are formed in day-21 oocytes
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• during in vitro maturation, oocytes isolated from day-21 mice and primed with PMSG for 48 h fail to undergo germinal vesicle breakdown; no metaphase II eggs are obtained after the superovulation treatment
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