Phenotypes associated with this allele

• Allele Symbol: Ap2a2^tm1.1Momk
• Allele Name: targeted mutation 1.1, Magdalene K Montgomery
• Allele ID: MGI:7612025
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Ap2a2^tm1.1Momk/Ap2a2^tm1.1Momk Tg(Adipoq-cre)#Pesch/0	involves: C57BL/6J * C57BL/6N * FVB	MGI:8234785

Genotype
MGI:8234785

cn1

• Allelic Composition: Ap2a2^tm1.1Momk/Ap2a2^tm1.1Momk; Tg(Adipoq-cre)#Pesch/0
• Genetic Background: involves: C57BL/6J * C57BL/6N * FVB

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

decreased susceptibility to diet-induced obesity ( J:297689 )

• gain less weight on a high fat diet for 8 weeks (26% increase vs 50% increase for diet matched controls)

• remain lean after 12 weeks on a high fat diet

adipose tissue

abnormal brown adipose tissue morphology ( J:297689 )

• brown adipose tissue is denser with fewer lipid droplets especially under high fat diet conditions

decreased brown adipose tissue amount ( J:297689 )

• significant brown adipose tissue atrophy on either a normal chow or high fat diet

increased fat cell mitochondrial DNA content ( J:297689 )

• a 30% increase in mtDNA content normalized to nuclear DNA content in inguinal white adipose tissue from mice on a high fat diet compared to diet matched controls

decreased white fat cell size ( J:297689 )

• decrease in adipocyte diameters but no change in numbers in epididymal white adipose tissue

decreased epididymal fat pad weight ( J:297689 )

• in mice on a high fat diet compared to diet matched controls

decreased inguinal fat pad weight ( J:297689 )

• in mice on a high fat diet compared to diet matched controls

abnormal white adipose tissue physiology ( J:297689 )

• superoxide generation is enhanced in adipocytes isolated from inguinal white adipose tissue on a normal chow diet

• superoxide generation in adipocytes from mice on a high fat diet is similar to wild-type cells from diet matched mice

homeostasis/metabolism

decreased fatty acid oxidation ( J:297689 )

• decrease palmitate oxidation in epididymal what adipose tissue explants from mice on a normal chow diet compared to diet matched controls

increased fatty acid oxidation ( J:297689 )

• increased in palmitate oxidation in inguinal white adipose tissue and brown adipose tissue explants from mice on a high fat diet compared to diet matched controls

• however, palmitate oxidation in explants from muscle or epididymal what adipose tissue is similar to wild-type controls

impaired adaptive thermogenesis ( J:297689 )

• cold intolerant independent of diet

decreased fasting circulating glucose level ( J:297689 )

• on a high fat diet

decreased circulating insulin level ( J:297689 )

• on a high fat diet

decreased circulating leptin level ( J:297689 )

• in high fat diet fed mice compared to diet matched controls

decreased circulating free fatty acids level ( J:297689 )

• decreased by 40% in mice on a high fat diet

abnormal energy homeostasis ( J:297689 )

• when on a high fat diet remain reliant on fatty acid (FA) utilization in a thermoneutral environment

increased energy expenditure ( J:297689 )

• in mice on a high fat diet compared to diet matched controls

decreased susceptibility to diet-induced obesity ( J:297689 )

• gain less weight on a high fat diet for 8 weeks (26% increase vs 50% increase for diet matched controls)

• remain lean after 12 weeks on a high fat diet

increased carbon dioxide production ( J:297689 )

• increased VCO2 at night and during the day in mice on a high fat diet compared to diet matched controls

increased oxygen consumption ( J:297689 )

• increased VO2 at night and during the day when on a high fat diet compared to diet matched controls

• in inguinal white adipose tissue explants from mice on a high fat diet oxygen consumption normalized to nuclear DNA content is increased 2-fold

• in epididymal white adipose tissue explants from mice on a high fat diet in the presence of pyruvate or FCCP oxygen consumption rate is increased

improved glucose tolerance ( J:297689 )

• on a high fat diet

increased insulin sensitivity ( J:297689 )

• after 12 or 21 weeks on a high fat diet

cellular

increased fat cell mitochondrial DNA content ( J:297689 )

• a 30% increase in mtDNA content normalized to nuclear DNA content in inguinal white adipose tissue from mice on a high fat diet compared to diet matched controls

increased mitochondrial number ( J:297689 )

• increased mitochondrial volume density is elevated more than 2 fold in inguinal white adipose tissue from high fat diet fed mice compared to diet matched controls

• however, there is no sign of damaged or swollen mitochondria

abnormal mitochondrial physiology ( J:297689 )

• enhanced mitochondrial uncoupled respiration in cultured adipocytes from mice on a normal chow or high fat diet

abnormal mitochondrial biogenesis ( J:297689 )

• increased induction of mitochondrial biogenesis in inguinal white adipose tissue explants from mice on a high fat diet compared to diet matched controls

decreased fatty acid oxidation ( J:297689 )

• decrease palmitate oxidation in epididymal what adipose tissue explants from mice on a normal chow diet compared to diet matched controls

increased fatty acid oxidation ( J:297689 )

• increased in palmitate oxidation in inguinal white adipose tissue and brown adipose tissue explants from mice on a high fat diet compared to diet matched controls

• however, palmitate oxidation in explants from muscle or epididymal what adipose tissue is similar to wild-type controls

immune system

decreased inflammatory response ( J:297689 )

• on a high fat diet display reduced diet-induced adipose tissue inflammation

liver/biliary system

decreased susceptibility to diet-induced hepatic steatosis ( J:297689 )

• when on a high fat diet