Ap2a2^tm1.1Momk
Targeted Allele Detail

Summary

• Symbol: Ap2a2^tm1.1Momk
• Name: adaptor-related protein complex 2, alpha 2 subunit; targeted mutation 1.1, Magdalene K Montgomery
• MGI ID: MGI:7612025
• Synonyms: Ap2a2^fl
• Gene: Ap2a2 Location: Chr7:141142093-141212924 bp, + strand Genetic Position: Chr7, 86.94 cM
• Alliance: Ap2a2^tm1.1Momk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:297689
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 141178192 of Chromosome 7 upstream of the exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 4 at position 141179040. Exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp mediated recombination. Subsequent cre expression results in a knockout mouse. (J:173534, J:297689)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ap2a2 Mutation: 76 strains or lines available

References

• Original: J:297689 Montgomery MK, et al., The role of Ap2a2 in PPARalpha-mediated regulation of lipolysis in adipose tissue. FASEB J. 2019 Dec;33(12):13267-13279
• All: 2 reference(s)