Phenotypes associated with this allele

• Allele Symbol: Lamp5^em1Lmit
• Allele Name: endonuclease-mediated mutation 1, Lars M Ittner
• Allele ID: MGI:7608054
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Lamp5^em1Lmit/Lamp5^em1Lmit	C57BL/6-Lamp5^em1Lmit	MGI:7608056
ht2	Lamp5^em1Lmit/Lamp5^+	C57BL/6-Lamp5^em1Lmit	MGI:7608057
cx3	Lamp5^em1Lmit/Lamp5^em1Lmit Tg(Thy1-MAPT*P301S)58.2Npa/0	involves: C57BL/6 * BALB/c	MGI:7608061
cx4	Lamp5^em1Lmit/Lamp5^+ Tg(Thy1-APP)3Somm/0	involves: C57BL/6 * C57BL/6J * DBA/2	MGI:7608059
cx5	Lamp5^em1Lmit/Lamp5^em1Lmit Tg(Thy1-APP)3Somm/0	involves: C57BL/6 * C57BL/6J * DBA/2	MGI:7608060

Genotype
MGI:7608056

hm1

• Allelic Composition: Lamp5^em1Lmit/Lamp5^em1Lmit
• Genetic Background: C57BL/6-Lamp5^em1Lmit

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

increased susceptibility to pharmacologically induced seizures ( J:345459 )

• mice exhibit an increase in severity and reduced latency of pentylenetetrazole-induced excitotoxic seizure than in heterozygotes or wild-type mice

abnormal habituation to a new environment ( J:345459 )

• mice show delayed habituation in the open field task

• however, no hyperactivity is seen

nervous system

increased susceptibility to pharmacologically induced seizures ( J:345459 )

• mice exhibit an increase in severity and reduced latency of pentylenetetrazole-induced excitotoxic seizure than in heterozygotes or wild-type mice

abnormal brain interneuron morphology ( J:345459 )

• when injected with an adeno-associated virus encoding a Lamp5 promoter-driven GFP reporter, mice show normal development of LAMP5+ interneurons at 1 months of age, however by 3-months of age, mice show a loss of LAMP5+ interneurons and dystrophic neurites in the cortex and dentate gyrus

neurite dystrophy ( J:345459 )

• when injected with an adeno-associated virus encoding a Lamp5 promoter-driven GFP reporter, mice show normal development of LAMP5+ interneurons at 1 months of age, however by 3-months of age, mice show dystrophic neurites in the cortex and dentate gyrus

abnormal brain wave pattern ( J:345459 )

• hippocampal EEG recordings show frequent spontaneous discharge events and increased high frequency power

• spectral power analysis shows increased high frequency power compared to controls

mortality/aging

mortality/aging ( J:345459 )

N • mice exhibit normal survival

Genotype
MGI:7608057

ht2

• Allelic Composition: Lamp5^em1Lmit/Lamp5⁺
• Genetic Background: C57BL/6-Lamp5^em1Lmit

behavior/neurological

increased susceptibility to pharmacologically induced seizures ( J:345459 )

• mice exhibit an increase in severity and reduced latency of pentylenetetrazole-induced excitotoxic seizure than in wild-type mice

abnormal habituation to a new environment ( J:345459 )

• mice show delayed habituation in the open field task

• however, no hyperactivity is seen

nervous system

increased susceptibility to pharmacologically induced seizures ( J:345459 )

• mice exhibit an increase in severity and reduced latency of pentylenetetrazole-induced excitotoxic seizure than in wild-type mice

mortality/aging

mortality/aging ( J:345459 )

N • mice exhibit normal survival

Genotype
MGI:7608061

cx3

• Allelic Composition: Lamp5^em1Lmit/Lamp5^em1Lmit; Tg(Thy1-MAPT*P301S)58.2Npa/0
• Genetic Background: involves: C57BL/6 * BALB/c

mortality/aging

mortality/aging ( J:345459 )

N • mice show similar survival as single Tg(Thy1-MAPT*P301S)58.2Npa hemizygotes or single Lamp5^em1Lmit homozygotes

behavior/neurological

impaired spatial learning ( J:345459 )

• mice show impaired learning in the Morris water maze compared to single Tg(Thy1-MAPT*P301S)58.2Npa hemizygotes

abnormal spatial reference memory ( J:345459 )

• mice show impaired memory in the Morris water maze compared to single Tg(Thy1-MAPT*P301S)58.2Npa hemizygotes

nervous system

abnormal brain wave pattern ( J:345459 )

• EEG recording shows increased numbers of spontaneous discharges and elevated high frequency spectral power while frequency of spectral power (theta) is reduced and theta/gamma cross frequency coupling, an EEG measure linked to memory, is compromised compared to single Tg(Thy1-MAPT*P301S)58.2Npa hemizyotes

Genotype
MGI:7608059

cx4

• Allelic Composition: Lamp5^em1Lmit/Lamp5⁺; Tg(Thy1-APP)3Somm/0
• Genetic Background: involves: C57BL/6 * C57BL/6J * DBA/2

mortality/aging

premature death ( J:345459 )

• survival is reduced similarly to that seen in single Tg(Thy1-APP)3Somm hemizygotes until 150 days and thereafter shows a trend towards increased mortality

behavior/neurological

impaired spatial learning ( J:345459 )

• learning in the Morris water maze is impaired compared to single Tg(Thy1-APP)3Somm hemizygotes at 12 months of age

abnormal spatial reference memory ( J:345459 )

• memory formation in the Morris water maze is impaired compared to single Tg(Thy1-APP)3Somm hemizygotes at 12 months of age

homeostasis/metabolism

amyloid beta deposits ( J:345459 )

• amyloid beta formation and plaque load in aged mice is similar to single Tg(Thy1-APP)3Somm hemizygotes

nervous system

amyloid beta deposits ( J:345459 )

• amyloid beta formation and plaque load in aged mice is similar to single Tg(Thy1-APP)3Somm hemizygotes

abnormal brain wave pattern ( J:345459 )

• spectral power at gamma (30-100 Hz) frequency is increased more than in single Tg(Thy1-APP)3Somm hemizygotes, indicating neuronal network hypersynchronicity

• theta/gamma cross frequency coupling, an EEG measure linked to memory, is disrupted more than in single Tg(Thy1-APP)3Somm hemizygotes

Genotype
MGI:7608060

cx5

• Allelic Composition: Lamp5^em1Lmit/Lamp5^em1Lmit; Tg(Thy1-APP)3Somm/0
• Genetic Background: involves: C57BL/6 * C57BL/6J * DBA/2

mortality/aging

premature death ( J:345459 )

• mice exhibit earlier and more rapid mortality and poor overall survival compared to single Tg(Thy1-APP)3Somm hemizygotes

behavior/neurological

hyperactivity ( J:345459 )

• mice show increased hyperactivity over the entire test period compared to single Tg(Thy1-APP)3Somm hemizygotes

• memory could not be tested due to high premature mortality rate

nervous system

abnormal brain wave pattern ( J:345459 )

• hippocampal EEG recordings show a further increase in spontaneous discharges and spike trains than seen in single Tg(Thy1-APP)3Somm hemizygotes

• spectral power at gamma (30-100 Hz) frequency is increased more than in single Tg(Thy1-APP)3Somm hemizygotes or Lamp5^em1Lmit/Lamp5⁺ Tg(Thy1-APP)3Somm/0 mice, indicating neuronal network hypersynchronicity

• theta/gamma cross frequency coupling, an EEG measure linked to memory, is disrupted more than in single Tg(Thy1-APP)3Somm hemizygotes