All Phenotypes Adad2<em2Osb> MGI Mouse

decreased male germ cell number ( J:337548 )

• elongated spermatids are rarely found in the seminiferous tubules

multinucleated giant male germ cells ( J:337548 )

• multinucleated cysts containing degenerated round spermatids are observed

decreased testis weight ( J:337548 )

• testis weight is significantly lower than that in age-matched heterozygous controls

abnormal spermatogenesis ( J:337548 )

• spermatogenesis is generally normal until the early round spermatid stage

arrest of spermiogenesis ( J:337548 )

• elongated spermatids are rarely found in the seminiferous tubules, and multinucleated cysts containing degenerated round spermatids are observed

• at ~P24 (around the onset of spermiogenesis) cluster-derived pachytene PIWI-interacting RNA (piRNAs) are reduced while transposon-derived (intron-, SINE-, LINE-, and LTR-derived) piRNAs are increased in the testes

• both MILI-bound and MIWI-bound pachytene cluster-derived piRNAs are reduced

• LINE-1 expression is not detected in testes, indicating that arrested spermiogenesis is not due to LINE-1 derepression

male infertility ( J:337548 )

• male mice bred with wild-type females for 8 weeks fail to sire any offspring