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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Lyz2em1(icre)Gpt
endonuclease-mediated mutation 1, GemPharmatech Co., Ltd
MGI:6729910
Summary 3 genotypes


Genotype
MGI:8221228
cn1
Allelic
Composition
Apoeem1Gpt/Apoeem1Gpt
Lyz2em1(icre)Gpt/Lyz2+
Slc16a3em1Gpt/Slc16a3em1Gpt
Genetic
Background
C57BL/6JGpt-Apoeem1Gpt Lyz2em1(icre)Gpt Slc16a3em1Gpt
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Apoeem1Gpt mutation (0 available); any Apoe mutation (163 available)
Lyz2em1(icre)Gpt mutation (0 available); any Lyz2 mutation (42 available)
Slc16a3em1Gpt mutation (0 available); any Slc16a3 mutation (23 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• mice fed a high fat diet (HFD) for 12 weeks show a significant reduction in lipid content, necrotic core, atherosclerotic plaque area and mRNA expression of Itgam (integrin alpha M, aka Cd11b) in the plaque, but greater aortic collagen content than HFD-fed mice homozygous only for Apoeem1Gpt and Slc16a3em1Gpt, suggesting a delay in atherosclerosis progression
• arteries of HFD-fed mice show a further elevation of the lactylation modifier EP300 (E1A binding protein p300), pan-lysine lactylation (Pan Kla), and H3 lysine 18 lactylation (H3K18la) with increased macrophage H3K18la in the aortic root relative to HFD-fed mice only homozygous for Apoeem1Gpt and Slc16a3em1Gpt
• arteries from HFD-fed mice show decreased mRNA expression of proinflammatory genes (Il6, Il1b, Tnf) and increased mRNA expression of anti-inflammatory genes (Arg1, Il10, Tgfb1) relative to arteries from HFD-fed mice only homozygous for Apoeem1Gpt and Slc16a3em1Gpt
• after treatment with C646 (an EP300 inhibitor), HFD-fed mice show a significant increase in atherosclerotic plaque area along with a reduction in the expression of H3K18la and of reparative genes (Arg1, Il10, Mrc1, Tgfb1, Pdha1, Idh2, Sdha, and Fh1) relative to vehicle-treated HFD-fed controls

immune system
• bone marrow-derived macrophages (BMDMs) from HFD-fed mice show increased cellular oxygen consumption rate (OCR) and decreased extracellular acidification rate (ECAR) relative to BMDMs from HFD-fed mice only homozygous for Apoeem1Gpt and Slc16a3em1Gpt
• HFD-fed mice show significantly higher serum IL-10 levels than HFD-fed mice only homozygous for Apoeem1Gpt and Slc16a3em1Gpt
• HFD-fed mice show significantly lower serum IL-6 levels than HFD-fed mice only homozygous for Apoeem1Gpt and Slc16a3em1Gpt
• HFD-fed mice show significantly lower serum TNF-alpha levels than HFD-fed mice only homozygous for Apoeem1Gpt and Slc16a3em1Gpt

cellular
• arteries from HFD-fed mice show decreased mRNA expression of glycolysis genes (Hk2 and Ldha) relative to arteries from HFD-fed mice only homozygous for Apoeem1Gpt and Slc16a3em1Gpt
• BMDMs from HFD-fed mice show a significant increase in the enrichment of H3 lysine 18 lactylation (H3K18la) at the promoters of reparative genes, including anti-inflammatory genes and TCA cycle genes (Arg1, Il10, Mrc1, Tgfb1, Pdha1, Idh2, Sdha, and Fh1) relative to BMDMs from HFD-fed mice only homozygous for Apoeem1Gpt and Slc16a3em1Gpt
• after treatment with C646 (an EP300 inhibitor), BMDMs from HFD-fed mice show a significant reduction in the enrichment of H3K18la at macrophage reparative gene promoters
• BMDMs from HFD-fed mice show increased cellular oxygen consumption rate (OCR) and decreased extracellular acidification rate (ECAR) relative to BMDMs from HFD-fed mice only homozygous for Apoeem1Gpt and Slc16a3em1Gpt
• moreover, arteries from HFD-fed mice show decreased mRNA expression of glycolysis genes and increased mRNA expression of tricarboxylic acid (TCA) cycle genes, suggesting improved mitochondria function
• arteries from HFD-fed mice show increased mRNA expression of tricarboxylic acid (TCA) cycle genes (Pdha1, Idh2, Sdha, and Fh1) relative to arteries from HFD-fed mice only homozygous for Apoeem1Gpt and Slc16a3em1Gpt

homeostasis/metabolism
• HFD-fed mice show significantly higher serum IL-10 levels than HFD-fed mice only homozygous for Apoeem1Gpt and Slc16a3em1Gpt
• HFD-fed mice show significantly lower serum IL-6 levels than HFD-fed mice only homozygous for Apoeem1Gpt and Slc16a3em1Gpt
• HFD-fed mice show significantly lower serum TNF-alpha levels than HFD-fed mice only homozygous for Apoeem1Gpt and Slc16a3em1Gpt
• HFD-fed mice show significantly higher serum TGF-beta levels than HFD-fed mice only homozygous for Apoeem1Gpt and Slc16a3em1Gpt

hematopoietic system
• bone marrow-derived macrophages (BMDMs) from HFD-fed mice show increased cellular oxygen consumption rate (OCR) and decreased extracellular acidification rate (ECAR) relative to BMDMs from HFD-fed mice only homozygous for Apoeem1Gpt and Slc16a3em1Gpt




Genotype
MGI:7410887
cn2
Allelic
Composition
Arih1em3Gpt/Arih1em3Gpt
Lyz2em1(icre)Gpt/Lyz2+
Genetic
Background
C57BL/6JGpt-Arih1em3Gpt Lyz2em1(icre)Gpt
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Arih1em3Gpt mutation (0 available); any Arih1 mutation (33 available)
Lyz2em1(icre)Gpt mutation (0 available); any Lyz2 mutation (42 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging

immune system
N
• in vitro differentiation of bone marrow derived dendritic cells and bone marrow derived macrophages is normal
• in bone marrow derived dendritic cells exposed to HSV-1 or cytoplasmic DNA
• however, bone marrow derived dendritic cells exhibit normal production of IFN-beta in response to SeV-, poly(I:C)-, or cGAMP
• in bone marrow derived dendritic cells exposed to HSV-1 or cytoplasmic DNA
• however, bone marrow derived dendritic cells exhibit normal production of IL6 in response to SeV-, poly(I:C)-, or cGAMP
• bone marrow derived dendritic cells exposed to HSV-1 or cytoplasmic DNA produce less IFN-beta and IL6
• HSV-1-infected mice exhibit increased viral titer in the brain, decreased IFN-beta and IL6 sera levels, and increased mortality compared with control mice
• however, bone marrow derived dendritic cells exhibit normal production of IFN-beta and IL6 in response to SeV-, poly(I:C)-, or cGAMP

homeostasis/metabolism




Genotype
MGI:7410894
cn3
Allelic
Composition
Arih1em3Gpt/Arih1em3Gpt
Lyz2em1(icre)Gpt/Lyz2+
Trex1em1Gpt/Trex1em1Gpt
Genetic
Background
C57BL/6JGpt-Arih1em3Gpt Trex1em1Gpt Lyz2em1(icre)Gpt
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Arih1em3Gpt mutation (0 available); any Arih1 mutation (33 available)
Lyz2em1(icre)Gpt mutation (0 available); any Lyz2 mutation (42 available)
Trex1em1Gpt mutation (0 available); any Trex1 mutation (28 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
N
• unlike Trex1em1Gpt homozygotes, mice exhibit normal survival

immune system
N
• unlike Trex1em1Gpt homozygotes, mice exhibit normalized spleen size and weight; serum levels of TNF, CXCL1, CCL5, and total IgG; and autoimmune phenotypes

growth/size/body
N
• unlike Trex1em1Gpt homozygotes, mice exhibit normalized development and body weight





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last database update
07/22/2025
MGI 6.24
The Jackson Laboratory