Analysis Tools
reproductive system
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• adult ovaries lack oocytes
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• newborn ovaries show severely impaired assembly of P-body-like granules; the mean area of DCP1A foci is reduced to 15% of that in control ovaries; however, ELAVL2 is robustly expressed in oocytes
• immunostaining of CDH1 (an oocyte marker) together with LAMININ showed direct connection between oocytes, indicating that cyst breakdown is severely impaired
• no nuclear translocation of FOXO3A is observed at P1; instead FOXO3A remains in the cytoplasm of P7 ovaries indicating that PI3K-AKT signaling is abnormally activated
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• at P7 and P21, the number of growing follicles, including follicles in the transition state, primary follicles and secondary follicles, is significantly lower than that in control ovaries
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• at P7 and P21, the number of primary ovarian follicles is severely reduced
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• at P7 and P21, the number of secondary ovarian follicles is severely reduced
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• at P21, the number of antral follicles is severely reduced
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• at P7, approximately half of the oocytes remain in cysts, unlike in control ovaries where most oocytes have developed to primordial follicles
• at P14, primordial follicles appear abnormally enlarged; the diameter of oocytes in primordial follicles is increased 1.3-fold
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• although cyst breakdown is compromised, a substantial number of primordial follicles is detected at P7
• at P21, the number of primordial follicles is severely reduced
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• no pups are born when female mice are crossed with wild-type males from 6 to 24 weeks after birth
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cellular
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• adult ovaries lack oocytes
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endocrine/exocrine glands
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• newborn ovaries show severely impaired assembly of P-body-like granules; the mean area of DCP1A foci is reduced to 15% of that in control ovaries; however, ELAVL2 is robustly expressed in oocytes
• immunostaining of CDH1 (an oocyte marker) together with LAMININ showed direct connection between oocytes, indicating that cyst breakdown is severely impaired
• no nuclear translocation of FOXO3A is observed at P1; instead FOXO3A remains in the cytoplasm of P7 ovaries indicating that PI3K-AKT signaling is abnormally activated
|
|
|
• at P7 and P21, the number of growing follicles, including follicles in the transition state, primary follicles and secondary follicles, is significantly lower than that in control ovaries
|
|
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• at P7 and P21, the number of primary ovarian follicles is severely reduced
|
|
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• at P7 and P21, the number of secondary ovarian follicles is severely reduced
|
|
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• at P21, the number of antral follicles is severely reduced
|
|
|
• at P7, approximately half of the oocytes remain in cysts, unlike in control ovaries where most oocytes have developed to primordial follicles
• at P14, primordial follicles appear abnormally enlarged; the diameter of oocytes in primordial follicles is increased 1.3-fold
|
|
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• although cyst breakdown is compromised, a substantial number of primordial follicles is detected at P7
• at P21, the number of primordial follicles is severely reduced
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