Ddx6^em1.1Ysa
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ddx6^em1.1Ysa
• Name: DEAD-box helicase 6; endonuclease-mediated mutation 1.1, Yumiko Saga
• MGI ID: MGI:6720237
• Synonyms: Ddx6^flox deltaNeo
• Gene: Ddx6 Location: Chr9:44516189-44552028 bp, + strand Genetic Position: Chr9, 24.84 cM, cytoband B
• Alliance: Ddx6^em1.1Ysa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:282136
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: By using CRISPR/Cas9 technology in ES cells, the sequence including exon 5 (ENSMUSE00000216525) was flanked by loxP sequences and was followed by a Pgk-neo cassette flanked by Frt sequences. The Pgk-neo cassette was removed by crossing with Rosa-Flp mice. (J:282136)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ddx6 Mutation: 59 strains or lines available

References

• Original: J:282136 Kato Y, et al., ELAVL2-directed RNA regulatory network drives the formation of quiescent primordial follicles. EMBO Rep. 2019 Dec 5;20(12):e48251
• All: 3 reference(s)