Phenotypes associated with this allele

• Allele Symbol: Prdx5^{tm1.1(KOMP)Vlcg}
• Allele Name: targeted mutation 1.1, Velocigene
• Allele ID: MGI:6719042
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Prdx5^tm1.1(KOMP)Vlcg/Prdx5^tm1.1(KOMP)Vlcg	involves: C57BL/6J * C57BL/6NTac	MGI:6719071

Genotype
MGI:6719071

hm1

• Allelic Composition: Prdx5^{tm1.1(KOMP)Vlcg}/Prdx5^{tm1.1(KOMP)Vlcg}
• Genetic Background: involves: C57BL/6J * C57BL/6NTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:271141 )

N • mice exhibit normal immune system development, with no significant alterations in the numbers and percentages of B and T lymphocytes and myeloid cells in primary and secondary lymphoid organs relative to wild-type controls

increased circulating interleukin-12b level ( J:271141 )

• at 65 h post-infection with Listeria monocytogenes, mice show a significantly higher serum IL-12 p40 level than similarly infected wild-type controls

• however, no differences in bacterial burden are observed in spleen or liver at 65 h post-infection

increased circulating tumor necrosis factor level ( J:271141 )

• at 36 h post-infection with Listeria monocytogenes, mice show a significantly higher serum TNF-alpha level than similarly infected wild-type controls

increased interleukin-12b secretion ( J:271141 )

• after stimulation with LPS and IFN-gamma, bone-marrow-derived dendritic cells (BMDCs) produce significantly more IL-12 p40 relative to wild-type BMDCs

increased interleukin-6 secretion ( J:271141 )

• after stimulation with LPS and IFN-gamma, BMDCs produce significantly more IL-6 relative to wild-type BMDCs

• pretreatment of BMDCs with tert-butylhydroquinone (tBHQ) reduces IL-6 production, unlike in wild-type BMDCs

increased tumor necrosis factor secretion ( J:271141 )

• after stimulation with LPS and IFN-gamma, BMDCs produce significantly more TNF-alpha relative to wild-type BMDCs

homeostasis/metabolism

increased circulating interleukin-12b level ( J:271141 )

• at 65 h post-infection with Listeria monocytogenes, mice show a significantly higher serum IL-12 p40 level than similarly infected wild-type controls

• however, no differences in bacterial burden are observed in spleen or liver at 65 h post-infection

increased circulating tumor necrosis factor level ( J:271141 )

• at 36 h post-infection with Listeria monocytogenes, mice show a significantly higher serum TNF-alpha level than similarly infected wild-type controls

abnormal nitric oxide homeostasis ( J:271141 )

• after stimulation with LPS and IFN-gamma, BMDCs produce significantly higher levels of nitrite relative to wild-type BMDCs, indicating increased reactive nitrogen intermediate (RNI) synthesis

• however, pretreatment of BMDCs with tert-butylhydroquinone (tBHQ, an electrophile that induces the antioxidant response) reduces NO production in stimulated BMDCs, as seen in wild-type BMDCs

decreased oxygen consumption ( J:271141 )

• BMDCs exhibit a reduced basal oxygen consumption rate (OCR) and a corresponding reduction in maximal respiration rate

cellular

abnormal cellular respiration ( J:271141 )

• BMDCs exhibit a reduced basal oxygen consumption rate (OCR) and a corresponding reduction in maximal respiration rate

abnormal mitochondrial ATP synthesis coupled electron transport ( J:271141 )

• BMDCs show reduced levels of cellular ATP corresponding to decreased mitochondrial respiration

• however, glycolysis and fatty acid oxidation are normal

abnormal redox activity ( J:271141 )

• BMDCs show lower mitochondrial reactive oxygen species (mROS) production both at basal state and after stimulation with LPS and IFN-gamma, as determined by MitoSOX, a superoxide-specific fluorescent probe that localizes to mitochondria

• however, unstimulated BMDCs show normal cellular redox states as determined by DCFDA, a redox-sensitive probe that detects several reactive oxygen and nitrogen intermediates

oxidative stress ( J:271141 )

• after stimulation with LPS and IFN-gamma, BMDCs show an elevated cellular oxidative state relative to wild-type BMDCs, as determined by DCFDA

• increase in oxidative stress is reduced to wild-type levels after iNOS inhibition with 1400W pretreatment, indicating that reactive nitrogen intermediate (RNI) production underlies the increase in overall oxidative stress in stimulated BMDCs