Phenotypes associated with this allele
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gcktm1Ydor mutation
(0 available);
any
Gck mutation
(59 available)
Tg(Ins1-cre/ERT)1Lphi mutation
(1 available)
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homeostasis/metabolism
endocrine/exocrine glands
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• tamoxifen-treated mice exhibit an increase in beta cell mass at 1.5 months of age, but this increase is no longer seen at 9 months of age
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gcktm1Ydor mutation
(0 available);
any
Gck mutation
(59 available)
Tg(Ins2-cre)25Mgn mutation
(2 available)
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homeostasis/metabolism
endocrine/exocrine glands
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• the increase in beta cell mass seen in high-fat/high-sugar diet-fed mice is attenuated in mutants
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• 62% increase in beta cell mass at 1.5 months of age
• the 62% increase in beta cell mass seen at 1.5 months of age is completely reversed by 8 months of age; the proportion of cre+ beta cells is decreased from 71% in young mice to 48% in older mice
• however, individual beta cell volume is unchanged
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• islets exhibit an increased membrane potential response at 2.8 mmol/l glucose
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• beta cells exhibit approximately a 2-fold increase in cell cycle entry accompanied by a 62% increase in beta cell mass at 1.5 months of age
• marker analysis indicates that with age beta cells show cellular stress, DNA damage and cell death over time
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• islets secrete more insulin per beta cell following glucose stimulation at 1.5 months and 8 months of age
• however, 8-month-old mice treated with diazoxide, an opener of the beta cell K-ATP channels and suppressor of endogenous insulin secretion, and subsequently administered insulin show a similar decrease in blood glucose levels as controls indicating no insulin resistance
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cellular