Phenotypes associated with this allele

• Allele Symbol: Trp63^tm3.1Aam
• Allele Name: targeted mutation 3.1, Alea A Mills
• Allele ID: MGI:6477383
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Trp63^tm3.1Aam/Trp63^tm3.1Aam	involves: 129S7/SvEvBrd * BALB/c * C57BL/6	MGI:6477395
ht2	Trp63^tm3.1Aam/Trp63^+	involves: 129S7/SvEvBrd * BALB/c * C57BL/6	MGI:6477398
ht3	Trp63^tm2.1Aam/Trp63^tm3.1Aam	involves: 129S7/SvEvBrd * BALB/c * C57BL/6	MGI:6477408

Genotype
MGI:6477395

hm1

• Allelic Composition: Trp63^tm3.1Aam/Trp63^tm3.1Aam
• Genetic Background: involves: 129S7/SvEvBrd * BALB/c * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

perinatal lethality, complete penetrance ( J:294158 )

embryo

abnormal embryo development ( J:294158 )

• defects in epithelial morphogenesis

integument

abnormal skin development ( J:294158 )

• neonates lack a mature skin barrier

limbs/digits/tail

abnormal limb morphology ( J:294158 )

• neonates exhibit severe limb defects including limb truncations

short limbs ( J:294158 )

• limb truncations

Genotype
MGI:6477398

ht2

• Allelic Composition: Trp63^tm3.1Aam/Trp63⁺
• Genetic Background: involves: 129S7/SvEvBrd * BALB/c * C57BL/6

craniofacial

cleft palate ( J:294158 )

• 2.3% of neonates exhibit cleft palate

cellular

decreased keratinocyte proliferation ( J:294158 )

• primary keratinocytes exhibit reduced proliferation

digestive/alimentary system

cleft palate ( J:294158 )

• 2.3% of neonates exhibit cleft palate

growth/size/body

cleft palate ( J:294158 )

• 2.3% of neonates exhibit cleft palate

integument

abnormal keratinocyte physiology ( J:294158 )

• primary keratinocytes exhibit reduced proliferation and a corresponding increases in cellular senescence

• however, no major skin abnormalities are seen at E16.5 or P0

decreased keratinocyte proliferation ( J:294158 )

• primary keratinocytes exhibit reduced proliferation

Genotype
MGI:6477408

ht3

• Allelic Composition: Trp63^tm2.1Aam/Trp63^tm3.1Aam
• Genetic Background: involves: 129S7/SvEvBrd * BALB/c * C57BL/6

craniofacial

cleft palate ( J:294158 )

• 70% of neonates exhibit cleft palate

digestive/alimentary system

cleft palate ( J:294158 )

• 70% of neonates exhibit cleft palate

growth/size/body

cleft palate ( J:294158 )

• 70% of neonates exhibit cleft palate

integument

thick dermal layer ( J:294158 )

• dermis is thicker in large areas of the skin

abnormal epidermal layer morphology ( J:294158 )

• epidermis is abnormal with the stratum basale and stratum spinosum affected

thick epidermis stratum basale ( J:294158 )

thick epidermis stratum spinosum ( J:294158 )

thick epidermis ( J:294158 )

• thickening of Trp63-, K14-, and K10-expressing layers in patches of the epidermis covering the skin surface

limbs/digits/tail

limbs/digits/tail phenotype ( J:294158 )

N • neonates do not exhibit limb defects