Analysis Tools
reproductive system
 N |
• female fertility and ovary size are similar to controls
|
azoospermia
(
J:291221
)
 |
• no spermatozoa are found in the cauda epididymis
|
|
|
• absence of post-meiotic round spermatids and elongated spermatids
|
|
|
• abnormal nuclei are seen in ~50% of zygotene spermatocytes
|
|
|
• increase in the number of apoptotic spermatocytes
|
|
|
• tubules contain numerous vacuoles
|
small testis
(
J:291221
)
|
|
• much smaller than controls at 8 weeks of age
|
|
|
• delay or arrest of spermatogenesis at the zygotene/pachytene transition
• defects in synapsis are seen including synapsis partner switch (different regions of one chromosome synapses with more than one partner with at least one partner being a non-homolog) and condensed zygotene (zygotene nuclei with highly condensed chromosomes similar to those seen in pachytene nuclei)
• in pachytene spermatocytes the number of homologous DNA crossovers is reduced with ~25% of autosomes lacking crossover foci
|
homeostasis/metabolism
|
|
• presence of foci labeled for the phosphorylated form of the histone variant H2AX indicate the presence of many unrepaired double strand breaks in mid/late pachytene and diplotene spermatocytes
|
endocrine/exocrine glands
|
|
• tubules contain numerous vacuoles
|
small testis
(
J:291221
)
|
|
• much smaller than controls at 8 weeks of age
|
cellular
azoospermia
(
J:291221
)
|
|
• no spermatozoa are found in the cauda epididymis
|
|
|
• absence of post-meiotic round spermatids and elongated spermatids
|
|
|
• abnormal nuclei are seen in ~50% of zygotene spermatocytes
|
|
|
• increase in the number of apoptotic spermatocytes
|
|
|
• presence of foci labeled for the phosphorylated form of the histone variant H2AX indicate the presence of many unrepaired double strand breaks in mid/late pachytene and diplotene spermatocytes
|
