Analysis Tools
mortality/aging
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• homozygous embryos are present in near Mendelian ratios at E7.5-E9.5 but absent at E12.5
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growth/size/body
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• at E8.5, head shape is sometimes abnormal; by E9.5, all homozygous embryos exhibit an abnormal head shape
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• only a minority of homozygous embryos are delayed at E8.5, with none categorized as delayed at E7.5
• by E9.5, all homozygous embryos are developmentally delayed
• however, all three germ layers are present and properly patterned at E8.5
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• at E8.5, homozygous embryos are always smaller than controls littermates
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embryo
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• by E9.5, all homozygous embryos are unturned
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• only a minority of homozygous embryos are delayed at E8.5, with none categorized as delayed at E7.5
• by E9.5, all homozygous embryos are developmentally delayed
• however, all three germ layers are present and properly patterned at E8.5
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• at E8.5, homozygous embryos are always smaller than controls littermates
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• by E9.5, most homozygous embryos have failed to close their cranial neural tube
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• at E8.5, most homozygous embryos exhibit an irregularly shaped allantois that is often blebby at the tip
• in most cases, the allantois has balled up at the tip of the tail by E9.5
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• at E9.5, placentas exhibit multiple labyrinth zone defects
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• at E9.5, the Tfeb+ labyrinth trophoblast layer fails to expand, remaining a thin layer
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• by E9.5, all homozygous embryos exhibit abnormal yolk sacs
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• at E9.5, Gcm1+ chorioallantoic branching is severely impaired
• however, all placentas appear properly developed at E8.5
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• at E9.5, six of 14 homozygous embryos show chorioallantoic attachment; however, less allantoic mesoderm is observed attached to the chorion relative to controls
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• at E9.5, eight of 14 homozygous embryos show failure of chorioallantoic attachment
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nervous system
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• by E9.5, most homozygous embryos have failed to close their cranial neural tube
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craniofacial
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• at E8.5, head shape is sometimes abnormal; by E9.5, all homozygous embryos exhibit an abnormal head shape
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