All Phenotypes Tg(tetO-Mir31)#Zhyu MGI Mouse

abnormal nursing ( J:271420 )

• doxycyline(dox)-treated females are incapable of nursing their pups, leading to neonatal lethality

increased mammary gland epithelial cell proliferation ( J:271420 )

• dox-treated mammary glands exhibit an increase in mammary epithelial cell proliferation

abnormal mammary gland morphology ( J:271420 )

• beta-casein is completely absent in dox-treated glands

abnormal mammary gland development ( J:271420 )

• dox-treated mice show mammary stem cell expansion at the expense of differentiation, showing a 4-fold larger population of Lin-CD24+CD29^high cells

• marker analysis of dox-treated mice indicates that differentiation of mammary epithelial cells is inhibited

• in vitro colony formation assay with purified CD24+CD29^high cells from dox-treated mice shows that the number of primary colonies that form is similar to controls but the numbers of secondary, tertiary, and subsequent colonies are increased indicating increased mammary stem cell self-renewal

• basal progenitor cells from dox-treated mice grown on feeder cell layers show an increase in the number of solid basal colonies at the cost of acinar colonies indicating an inhibition of luminal differentiation or suppression of luminal cell colony formation

abnormal mammary gland growth during pregnancy ( J:271420 )

• dox-treated mammary glands fail to form alveoli and retain undifferentiated ductal structures at post-pregnancy 14.5 and lactation day 1

abnormal mammary gland epithelium morphology ( J:271420 )

• marker analysis of dox-treated mice indicates that differentiation of mammary epithelial cells is inhibited

abnormal mammary gland duct morphology ( J:271420 )

• dox-treated mammary glands fail to form alveoli and retain undifferentiated ductal structures at post-pregnancy 14.5 and lactation day 1

abnormal branching of the mammary ductal tree ( J:271420 )

• dox-treated mice show mildly inhibited mammary branching and ductal elongation at 6 weeks of age, however by 10 weeks of age, these effects are no longer seen

• mammary glands of all dox-treated 12 week old mice show no tertiary branch development and varying defects in ductal elongation and branching, and the appearance of multiple terminal end bud-like structures

mammary gland duct hyperplasia ( J:271420 )

• dox-treated mice have thicker mammary ducts, characterized by an increase in cell layers, at 12 weeks of age

abnormal mammary gland alveolus morphology ( J:271420 )

• dox-treated mammary glands fail to form alveoli and retain undifferentiated ductal structures at post-pregnancy 14.5 and lactation day 1

mammary gland hyperplasia ( J:271420 )

increased mammary gland epithelial cell proliferation ( J:271420 )

• dox-treated mammary glands exhibit an increase in mammary epithelial cell proliferation

increased mammary gland epithelial cell proliferation ( J:271420 )

• dox-treated mammary glands exhibit an increase in mammary epithelial cell proliferation

abnormal mammary gland morphology ( J:271420 )

• beta-casein is completely absent in dox-treated glands

abnormal mammary gland development ( J:271420 )

• dox-treated mice show mammary stem cell expansion at the expense of differentiation, showing a 4-fold larger population of Lin-CD24+CD29^high cells

• marker analysis of dox-treated mice indicates that differentiation of mammary epithelial cells is inhibited

• in vitro colony formation assay with purified CD24+CD29^high cells from dox-treated mice shows that the number of primary colonies that form is similar to controls but the numbers of secondary, tertiary, and subsequent colonies are increased indicating increased mammary stem cell self-renewal

• basal progenitor cells from dox-treated mice grown on feeder cell layers show an increase in the number of solid basal colonies at the cost of acinar colonies indicating an inhibition of luminal differentiation or suppression of luminal cell colony formation

abnormal mammary gland growth during pregnancy ( J:271420 )

• dox-treated mammary glands fail to form alveoli and retain undifferentiated ductal structures at post-pregnancy 14.5 and lactation day 1

abnormal mammary gland epithelium morphology ( J:271420 )

• marker analysis of dox-treated mice indicates that differentiation of mammary epithelial cells is inhibited

abnormal mammary gland duct morphology ( J:271420 )

• dox-treated mammary glands fail to form alveoli and retain undifferentiated ductal structures at post-pregnancy 14.5 and lactation day 1

abnormal branching of the mammary ductal tree ( J:271420 )

• dox-treated mice show mildly inhibited mammary branching and ductal elongation at 6 weeks of age, however by 10 weeks of age, these effects are no longer seen

• mammary glands of all dox-treated 12 week old mice show no tertiary branch development and varying defects in ductal elongation and branching, and the appearance of multiple terminal end bud-like structures

mammary gland duct hyperplasia ( J:271420 )

• dox-treated mice have thicker mammary ducts, characterized by an increase in cell layers, at 12 weeks of age

abnormal mammary gland alveolus morphology ( J:271420 )

• dox-treated mammary glands fail to form alveoli and retain undifferentiated ductal structures at post-pregnancy 14.5 and lactation day 1

mammary gland hyperplasia ( J:271420 )

abnormal mammary gland growth during pregnancy ( J:271420 )

• dox-treated mammary glands fail to form alveoli and retain undifferentiated ductal structures at post-pregnancy 14.5 and lactation day 1

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

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