Phenotypes associated with this allele

• Allele Symbol: Inava^tm1.1Itl
• Allele Name: targeted mutation 1,1, inGenious Targeting Laboratory
• Allele ID: MGI:6356370
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Inava^tm1.1Itl/Inava^tm1.1Itl	involves: 129S6/SvEvTac * C57BL/6NTac	MGI:6514866

Genotype
MGI:6514866

hm1

• Allelic Composition: Inava^tm1.1Itl/Inava^tm1.1Itl
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6NTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

digestive/alimentary system

abnormal intestinal epithelium morphology ( J:257317 )

• mice exhibit defects in the intestinal epithelial cell barrier

• surface levels of E-cadherin are reduced in isolated colonic intestinal epithelial cells and organoid-derived monolayers

• colonic organoids show disorganized E-cadherin staining along the adherens junctions and increased puncta formation in the cytosol

• however, no differences are observed in the mRNA and protein levels or localization of other epithelial cell junction proteins (including occludin, ZO1, claudin 1 or claudin 2)

abnormal intestinal epithelium physiology ( J:257317 )

• colon and small intestine epithelial tissues show significantly increased permeability to smaller solutes such as Lucifer Yellow (0.4KDa), but not to larger solutes such as FITC-dextran (4KDa)

increased susceptibility to induced colitis ( J:257317 )

• mice exhibit impaired recovery from dextran sodium sulfate (DSS)-induced colitis as evidenced by an increased body weight loss, reduced colon length, and more severe colonic histopathology at day 13 after DSS treatment relative to wild-type controls

immune system

increased susceptibility to induced colitis ( J:257317 )

• mice exhibit impaired recovery from dextran sodium sulfate (DSS)-induced colitis as evidenced by an increased body weight loss, reduced colon length, and more severe colonic histopathology at day 13 after DSS treatment relative to wild-type controls

increased susceptibility to bacterial infection ( J:257317 )

• following challenge with Citrobacter rodentium, mice exhibit a higher bacterial load in stool and increased translocation of C. rodentium to the mesenteric lymph nodes and spleen at day 5 as well as shortened colon length and more severe histopathology (including crypt damage) at day 12 post-infection relative to wild-type controls

• however, cytokine response is not impaired at 12 days post-infection

homeostasis/metabolism

abnormal protein level ( J:257317 )

• intestinal epithelial cells isolated from the colon or small intestine show increased protein levels of cytohesin-1; epithelial monolayers derived from colonic organoids show increased levels of cytohesin-1 protein in both membrane and cytosolic protein fractions with no detectable changes in cytohesin-1 mRNA level

• organoid-derived intestinal epithelial monolayers show higher membrane-associated ARF6-GTP levels

cellular

increased cell migration ( J:257317 )

• organoid-derived colonic monolayers show a significantly increased migratory rate at baseline and during hepatocyte growth factor (HGF)-induced cell migration relative to wild-type controls