Phenotypes associated with this allele

• Allele Symbol: Iftap^{tm1b(KOMP)Wtsi}
• Allele Name: targeted mutation 1b, Wellcome Trust Sanger Institute
• Allele ID: MGI:6356351
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Iftap^tm1b(KOMP)Wtsi/Iftap^tm1b(KOMP)Wtsi	involves: C57BL/6J * C57BL/6N	MGI:6479003

Genotype
MGI:6479003

hm1

• Allelic Composition: Iftap^{tm1b(KOMP)Wtsi}/Iftap^{tm1b(KOMP)Wtsi}
• Genetic Background: involves: C57BL/6J * C57BL/6N
• Cell Lines: EPD0067_1_B06

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

reproductive system phenotype ( J:236765 , J:236765 , J:268060 )

N • mice exhibit normal male and female fertility under physiological conditions (J:236765)

♂N • males show normal testicular tissue architecture relative to controls (J:236765)

♂N • at 2-7 months of age, sexually mature males show no significant changes in testis weight, epididymal sperm count, sperm motility and morphology or sperm capacitation progress relative to control males (J:268060)

abnormal sperm head morphology ( J:268060 )

♂ • males show an increased number of sperm with abnormally shaped heads relative to control males (15.9% vs 6.5%, respectively)

♂ • however, the structures of the manchette and forming acrosome are grossly normal, indicating that sperm head shaping is unaffected

abnormal copulation ( J:268060 )

♂ • under non-standard mating conditions that mimic polyandry and introduce sperm competition, sperm fail to successfully compete with control sperm

reduced male fertility ( J:268060 )

♂ • males exhibit reduced fertility only in a competitive context but not under standard mating protocols

impaired fertilization ( J:268060 )

♂ • sperm exhibit a reduced ability to fertilize cumulus-intact eggs in vitro

♂ • however, sperm ability to penetrate the cumulus cells is normal

impaired acrosome reaction ( J:268060 )

♂ • sperm show reduced ability to undergo calcium ionophore (A23187)-induced acrosome reaction

cellular

abnormal sperm head morphology ( J:268060 )

♂ • males show an increased number of sperm with abnormally shaped heads relative to control males (15.9% vs 6.5%, respectively)

♂ • however, the structures of the manchette and forming acrosome are grossly normal, indicating that sperm head shaping is unaffected

mortality/aging

mortality/aging ( J:236765 )

N • mice are viable and show no obvious morphological, anatomical or physiological abnormalities up to 6 months of age