Iftap^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Iftap^{tm1b(KOMP)Wtsi}
• Name: intraflagellar transport associated protein; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6356351
• Synonyms: NWC-KO, NWC-KOMPcre
• Gene: Iftap Location: Chr2:101391124-101459376 bp, - strand Genetic Position: Chr2, 53.83 cM, cytoband E2
• Alliance: Iftap^{tm1b(KOMP)Wtsi} page
• IMPC: Iftap gene page

Mutation origin

• Mutant Cell Line: EPD0067_1_B06
• Germline Transmission: Earliest citation of germline transmission: J:236765
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 101413619 of Chromosome 2 upstream of critical exon 5 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of exon 5 at position 101414384. Exon 5 is thus flanked by loxP sites. A reporter knockout allele was created by cre recombinase expression in mice carrying the parental Iftap^{tm1a(KOMP)Wtsi} allele to remove loxP-flanked exon 5. RT-PCR and Western blot analysis confirmed the absence of full-length transcript and protein expression, respectively, in homozygous mutant testes. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:236765)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Iftap Mutation: 21 strains or lines available

References

• Original: J:236765 Kasztura M, et al., Search for the Function of NWC, Third Gene Within RAG Locus: Generation and Characterization of NWC-Deficient Mice. Arch Immunol Ther Exp (Warsz). 2016 Aug;64(4):311-9
• All: 2 reference(s)