Phenotypes associated with this allele

• Allele Symbol: Nlrp2^tm1.2Itl
• Allele Name: targeted mutation 1.2, Ingenious Targeting Laboratory
• Allele ID: MGI:6343489
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nlrp2^tm1.2Itl/Nlrp2^tm1.2Itl	involves: C57BL/6	MGI:6718825

Genotype
MGI:6718825

hm1

• Allelic Composition: Nlrp2^tm1.2Itl/Nlrp2^tm1.2Itl
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

embryo

abnormal preimplantation embryo development ( J:237496 )

• after parthenogenetic activation with strontium chloride, oocytes from 22-25-week-old mice progress asynchronously and with reduced rates through the 4-cell and morula stages; most embryos are lost or look fragmented by day 5 and less than 10% reach the blastocyst stage

• however, oocytes from 6-10-week-old mice show normal progression through the 2-cell, 4-cell, and morula stages and ~30% reach the blastocyst stage 5 d after parthenogenetic activation, similar to oocytes from age-matched wild-type mice

abnormal blastocyst formation ( J:237496 )

• after parthenogenetic activation, less than 10% of oocytes from 22-25-week-old mice develop to the blastocyst stage, unlike activated oocytes from aged-matched wild-type mice, ~ 80% of which develop into blastocysts

• however, oocytes from 6-10-week-old mice reach the blastocyst stage with normal efficiency and kinetics 5 days after parthenogenetic activation

reproductive system

reduced female fertility ( J:237496 )

♀ • marked decline in female fertility with progressing age

decreased litter size ( J:237496 )

• litters of mature adult mutant females are significantly smaller than those of wild-type females

cellular

cellular phenotype ( J:237496 )

N • metaphase II-arrested (MII) oocytes from both 6-10- and 22-25-week-old mice show normal spindle morphology and length

N • MII oocytes from mature adult mice show normal chromosome segregation after parthenogenetic activation

maternal effect ( J:237496 )

• in vitro, oocytes of mature adult females exhibit defective preimplantation embryo development after parthenogenetic activation

immune system

immune system phenotype ( J:237496 )

N • no alterations are observed in innate or adaptive immune responses in ex vivo-stimulated leukocytes and in in vivo-challenged mice