Analysis Tools
integument
| N |
• at E17.5, homozygotes show normal kinetics of epidermal barrier development, as determined by dye penetration assays
• at P5, skin barrier function is normal, as determined by trans-epidermal water loss (TEWL) and transdermal diffusion assays
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• at P3, the lower stratum corneum appears more compact than that in wild-type controls
• cornified envelopes isolated from neonatal skin are more sensitive to mechanical stress, with only 10% remaining intact after a 3-min sonication versus 50% of wild-type envelopes
• levels of profilaggrin and filaggrin monomers are reduced at P3 whereas amino acid components of the natural moisturizing factor are significantly increased at P5 in the superficial stratum corneum
• the electrophoretic mobility of involucrin (a cornified envelope precursor) is modified, suggesting post-translational modifications
• however, no alterations in corneocyte cohesion or desquamation are observed in the stratum corneum at P5
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• at P3, homozygotes show a deceased amount of keratohyalin granules in the stratum granulosum relative to wild-type controls; this was no longer visible after P8-10
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• at P3, keratohyalin granules are smaller than those in wild-type controls
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scaly skin
(
J:214662
)
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• homozygotes develop a transient scaly skin phenotype during the first week of life; this is no longer visible by P7-8, after hair growth occurs
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homeostasis/metabolism
| N |
• adult homozygotes show normal kinetics of wound closure after acute skin injury, with no differences in the inflammatory cells infiltrate in the epidermis during wound healing
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