Dmkn^tm1Jonca
Targeted Allele Detail

Summary

• Symbol: Dmkn^tm1Jonca
• Name: dermokine; targeted mutation 1, Nathalie Jonca
• MGI ID: MGI:6342886
• Synonyms: beta/gamma^-
• Gene: Dmkn Location: Chr7:30463181-30480488 bp, + strand Genetic Position: Chr7, 19.24 cM, cytoband A3
• Alliance: Dmkn^tm1Jonca page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:214662
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Modified isoform(s), Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exons 1 to 5 were replaced by a floxed neomycin cassette. Cre-mediated recombination removed the neo cassette. This strategy generated mice deficient in Dmkn beta and gamma isoforms, but still able to express the alpha isoform. Total absence of the beta and gamma isoforms was verified by RT-PCR analysis. Immunohistochemistry confirmed the absence of beta and gamma isoforms in the granular layer of neonatal epidermis in homozygous mutant mice. Quantitative RT-PCR revealed a significant increase in the expression of transcripts encoding the alpha isoform in the neonatal epidermis (8-fold) as well as in the ear skin (>3-fold) and tongue (>2-fold) of P15 homozygous mutant mice. (J:214662)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dmkn Mutation: 25 strains or lines available

References

• Original: J:214662 Leclerc EA, et al., Mice deficient for the epidermal dermokine beta and gamma isoforms display transient cornification defects. J Cell Sci. 2014 Jul 1;127(Pt 13):2862-72
• All: 1 reference(s)