Phenotypes associated with this allele

• Allele Symbol: Mettl23^em1Ogu
• Allele Name: endonuclease-mediated mutation 1, Atsuo Ogura
• Allele ID: MGI:6342883
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mettl23^em1Ogu/Mettl23^em1Ogu	involves: C57BL/6 * DBA/2	MGI:7439319

Genotype
MGI:7439319

hm1

• Allelic Composition: Mettl23^em1Ogu/Mettl23^em1Ogu
• Genetic Background: involves: C57BL/6 * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

decreased survivor rate ( J:254851 )

• only a few homozygotes survive to adulthood

postnatal lethality, incomplete penetrance ( J:254851 )

• although homozygotes are born alive, ~33% of newborn mice die before weaning from unknown causes

reproductive system

decreased litter size ( J:254851 )

• natural mating of homozygous pairs of mice results in a significantly smaller litter size than that obtained from mating of heterozygous pairs

cellular

abnormal DNA methylation ( J:254851 )

• conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) is impaired in maternal zygotes: the 5mC level remains high while the 5hmC level remains low in the male pronucleus; however, injection of Mettl23 mRNA into oocytes restores the 5mC to 5hmC conversion in maternal zygotes

• immunofluorescence analysis of Tet3 (a 5mC-oxidizing enzyme responsible for active DNA demethylation) shows a significantly reduced Tet3 signal in maternal zygotes; however, injection of Mettl23 mRNA into maternal zygotes restores the accumulation of Tet3 into the male pronucleus

• only faint signals for STPG4 (sperm-tail PG-rich repeat containing 4; aka GSE) are detected in the pronuclei of maternal zygotes; however, injection of Mettl23 mRNA results in recruitment of STPG4 into the male pronucleus, indicating that maternal Mettl23 is likely responsible for 5mC oxidation by recruiting the STPG4-Tet3 complex into the chromatin during zygote development