Analysis Tools
cellular
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• at E5.5, TUNEL+ cells are specifically detected in the epiblast
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embryo
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• embryos fail to initiate gastrulation by E7.5, as shown by complete lack of T (brachyury) expression
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• embryos do not progress morphologically past the E5.5 stage
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• embryos do implant and form an egg cylinder but are developmentally delayed
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• at E6.5, embryos are smaller but show normal pre-streak egg cylinder morphology
• at E7.5, embryos are much smaller than controls and show many morphological defects
• however, embryo size is normal at E5.5
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• at E7.5, the embryonic portion is much smaller than that in control embryos
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• at E5.5, the embryonic ectoderm appears slightly disorganized; however, epiblast cells properly express OCT4
• at E7.5, the epiblast largely remains as pseudostratified epithelium
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• at E5.5, TUNEL+ cells are specifically detected in the epiblast
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• at E5.5, the egg cylinder is slightly smaller but otherwise morphologically normal
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• no morphologically or molecularly marked mesoderm is detected at E7.5
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• no morphologically or molecularly marked primitive streak is detected at E7.5
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• no amniotic cavity is detected at E7.5
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• no ectoplacental cavity is detected at E7.5
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• no exocoelomic cavity is detected at E7.5
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• at E7.5, embryos show loosening/buckling of the visceral endoderm (VE); the VE appears to be losing contact with the epiblast and extraembryonic ectoderm resulting in a variable bubble-like cavity
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• at E7.5, it appears as if the visceral endoderm has continued to proliferate
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growth/size/body
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• embryos do implant and form an egg cylinder but are developmentally delayed
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• at E6.5, embryos are smaller but show normal pre-streak egg cylinder morphology
• at E7.5, embryos are much smaller than controls and show many morphological defects
• however, embryo size is normal at E5.5
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mortality/aging
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• embryos are recovered in Mendelian ratios at E7.5; however, no embryos are found at E9.5
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IMPC - JAX
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