Phenotypes associated with this allele

• Allele Symbol: Atp6v0a2^tm1Kdb
• Allele Name: targeted mutation 1, Kenneth D Beaman
• Allele ID: MGI:6303630
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Atp6v0a2^tm1Kdb/Atp6v0a2^tm1Kdb Tg(MMTV-cre)4Mam/0	involves: 129 * C57BL/6 * FVB	MGI:6303631
cn2	Atp6v0a2^tm1Kdb/Atp6v0a2^tm1Kdb Commd10^Tg(Vav1-icre)A2Kio/Commd10^+	involves: C57BL/6 * C57BL/10 * CBA/Ca	MGI:6303639

Genotype
MGI:6303631

cn1

• Allelic Composition: Atp6v0a2^tm1Kdb/Atp6v0a2^tm1Kdb; Tg(MMTV-cre)4Mam/0
• Genetic Background: involves: 129 * C57BL/6 * FVB

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

abnormal mammary gland development ( J:274047 )

♀ • glands from pubertal mice show delayed ductal outgrowth

impaired mammary gland growth during pregnancy ( J:274047 )

♀ • early pregnant mammary glands show decreased ductal elongation, bifurcation and tertiary branching

abnormal mammary gland epithelium morphology ( J:274047 )

♀ • mice and exhibit a disintegrated mammary epithelium, with a decrease in mammary epithelial content and in myoepithelial cells

abnormal mammary gland duct morphology ( J:274047 )

♀ • at puberty, mice show a decrease in relative ductal length

♀ • during early pregnancy, mice show decreased ductal elongation

abnormal branching of the mammary ductal tree ( J:274047 )

♀ • early pregnant mammary glands show decreased bifurcation and tertiary branching

abnormal mammary duct terminal end bud morphology ( J:274047 )

♀ • mice show a decrease in the number of terminal end buds and ductal branches

abnormal lactation ( J:274047 )

♀ • mice exhibit a decrease in expression of beta-casein, a lactation marker, and litters from mutant mice have lower body weight than litters from wild-type mice, suggesting abnormal lactation

integument

abnormal mammary gland development ( J:274047 )

♀ • glands from pubertal mice show delayed ductal outgrowth

impaired mammary gland growth during pregnancy ( J:274047 )

♀ • early pregnant mammary glands show decreased ductal elongation, bifurcation and tertiary branching

abnormal mammary gland epithelium morphology ( J:274047 )

♀ • mice and exhibit a disintegrated mammary epithelium, with a decrease in mammary epithelial content and in myoepithelial cells

abnormal mammary gland duct morphology ( J:274047 )

♀ • at puberty, mice show a decrease in relative ductal length

♀ • during early pregnancy, mice show decreased ductal elongation

abnormal branching of the mammary ductal tree ( J:274047 )

♀ • early pregnant mammary glands show decreased bifurcation and tertiary branching

abnormal mammary duct terminal end bud morphology ( J:274047 )

♀ • mice show a decrease in the number of terminal end buds and ductal branches

abnormal lactation ( J:274047 )

♀ • mice exhibit a decrease in expression of beta-casein, a lactation marker, and litters from mutant mice have lower body weight than litters from wild-type mice, suggesting abnormal lactation

reproductive system

impaired mammary gland growth during pregnancy ( J:274047 )

♀ • early pregnant mammary glands show decreased ductal elongation, bifurcation and tertiary branching

Genotype
MGI:6303639

cn2

• Allelic Composition: Atp6v0a2^tm1Kdb/Atp6v0a2^tm1Kdb; Commd10^{Tg(Vav1-icre)A2Kio}/Commd10⁺
• Genetic Background: involves: C57BL/6 * C57BL/10 * CBA/Ca

hematopoietic system

decreased T cell number ( J:274048 )

• population of lymphoid cells in spleen is severely diminished

• total T cells are reduced 4.8-fold and 16.6-fold in spleen and blood, respectively

decreased alpha-beta T cell number ( J:274048 )

• only the alpha-beta T cells, not the gamma-delta T cells, are diminished in the spleen and blood

• the alpha-beta T cell percentage is reduced 6.23-fold and cell number is reduced 21-fold in the spleen

• similar reductions in alpha-beta T cells is seen in the thymus and bone marrow

decreased CD4-positive, alpha-beta T cell number ( J:274048 )

• percentage of CD4+ T helper cells is reduced 25.71-fold in the spleen and 58.3-fold in the blood

abnormal CD8-positive, alpha-beta cytotoxic T cell morphology ( J:274048 )

• percentage of CD8+ cytotoxic T cells is reduced 13.73-fold in the spleen and 11.16-fold in the blood

immune system

decreased T cell number ( J:274048 )

• population of lymphoid cells in spleen is severely diminished

• total T cells are reduced 4.8-fold and 16.6-fold in spleen and blood, respectively

decreased alpha-beta T cell number ( J:274048 )

• only the alpha-beta T cells, not the gamma-delta T cells, are diminished in the spleen and blood

• the alpha-beta T cell percentage is reduced 6.23-fold and cell number is reduced 21-fold in the spleen

• similar reductions in alpha-beta T cells is seen in the thymus and bone marrow

decreased CD4-positive, alpha-beta T cell number ( J:274048 )

• percentage of CD4+ T helper cells is reduced 25.71-fold in the spleen and 58.3-fold in the blood

abnormal CD8-positive, alpha-beta cytotoxic T cell morphology ( J:274048 )

• percentage of CD8+ cytotoxic T cells is reduced 13.73-fold in the spleen and 11.16-fold in the blood

neoplasm

increased metastatic potential ( J:274048 )

• 14 days after tumor cell implantation, breast tumor cells metastasize to the lungs of mutant mice but not control mice

increased incidence of induced tumors ( J:274048 )

• mice implanted with a syngeneic tumor cell line E0771 in the 4th mammary foot pad show a faster rate of tumor growth and grow larger than in control mice implanted with tumor cells

• twice as many myeloid derived suppressor cells are present in the tumor microenvironment compared to controls

• fewer lymphoid cells are recruited to the tumor microenvironment compared to controls, with reduced CD3+CD19- T cell population due to reduced numbers of CD3+CD19-gamma-deltaTCR- alpha-beta T cells

• the tumor microenvironment shows a reduction in both CD4+ T helper cells and CD8+ cytotoxic T cell populations compared to controls

decreased tumor latency ( J:274048 )

• mice implanted with a syngeneic tumor cell line E0771 in the 4th mammary foot pad show a faster rate of tumor growth than in control mice implanted with tumor cells