All Phenotypes Ang<tm1Dats> MGI Mouse

abnormal common myeloid progenitor cell morphology ( J:236081 )

• common myeloid progenitors (CMPs) show less active cycling

abnormal granulocyte monocyte progenitor cell morphology ( J:236081 )

• granulocyte-macrophage progenitors (GMPs) show less active cycling

increased lymphocyte cell number ( J:236081 )

• mild lymphocytosis in 8-12 week old mice

decreased hematopoietic cell number ( J:236081 )

• 22 month old mice show a reduction in the number of primitive hematopoietic cells in the bone marrow

decreased leukocyte cell number ( J:236081 )

• 22 month old mice develop leukopenia

increased hematopoietic stem cell number ( J:236081 )

• bone marrow hematopoietic stem/progenitor cells (HSPCs) show a 2-fold increase in the number of long-term hematopoietic stem/progenitor cells (LT-HSCs), but not short term HSCs (St-HSCs) or multi-potent progenitors (MPPs)

abnormal megakaryocyte-erythroid progenitor cell morphology ( J:236081 )

• megakaryocyte-erythroid progenitors (MEPs) show less active cycling

abnormal hematopoietic system physiology ( J:236081 )

• myeloid-restricted progenitor (MyePro) cells, including common myeloid progenitors (CMPs), granulocyte-macrophage progenitor (GMP), and megakaryocyte-erythroid progenitor (MEP) cells show less active cycling

• common lymphoid progenitor (CLP) and pre-pro B cells cycle more actively and incorporate more BrdU

• myeloid-biased multi-potent progenitors (MPP3s) show restricted proliferation while lymphoid-biased multi-potent progenitors (MPP4s) show more active cycling

• LSK cells show a higher rate of protein synthesis while MyePro cells show a reduced rate of protein synthesis

abnormal hematopoietic stem cell physiology ( J:236081 )

• mice show elevated apoptosis across hematopoietic lineages

increased hematopoietic stem cell proliferation ( J:236081 )

• LT-HSCs exhibit a reduction in G0 phase and a corresponding increase in S/G2/M phases of the cell cycle and enhanced bromodeoxyuridine incorporation, indicating increased cycling and proliferation

• ST-HSCs and MPPs exhibit increased cycling but to a less severe degree than LT-HSCs

increased cellular sensitivity to gamma-irradiation ( J:236081 )

• mice exhibit reduced blood leukocyte recovery following exposure to various doses of gamma radiation

• mice show increased apoptosis in all primitive hematopoietic cell types and reduced lymphoid and myeloid colon formation in response to gamma irradiation

• pre-treatment with angiogenin (ANG) protects against radiation-induced loss of the total bone marrow number and various bone marrow cell subsets and restricts HSPC and LymPro cell cycling, prevents radiation-induced loss of MyePro cell number and promotes their proliferation, and protects against irradiation-induced apoptosis in primitive hematopoietic cells

increased hematopoietic stem cell proliferation ( J:236081 )

• LT-HSCs exhibit a reduction in G0 phase and a corresponding increase in S/G2/M phases of the cell cycle and enhanced bromodeoxyuridine incorporation, indicating increased cycling and proliferation

• ST-HSCs and MPPs exhibit increased cycling but to a less severe degree than LT-HSCs

increased mortality induced by gamma-irradiation ( J:236081 )

• mice exhibit reduced survival following exposure to various doses of gamma radiation

• pretreatment with angiogenin (ANG) before gamma irradiation improves survival rate from 20% to 90%

increased susceptibility to xenobiotic induced morbidity/mortality ( J:236081 )

• serial proliferative stress with weekly 5-fluorouracil injections result in excess mortality

increased physiological sensitivity to xenobiotic ( J:236081 )

• under stress conditions, progenitor response to the genotoxic agent, 5-fluorouracil is exaggerated