Phenotypes associated with this allele

• Allele Symbol: Rora^{tm1c(EUCOMM)Wtsi}
• Allele Name: targeted mutation 1c, Wellcome Trust Sanger Institute
• Allele ID: MGI:6256736
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Rora^tm1c(EUCOMM)Wtsi/Rora^tm1c(EUCOMM)Wtsi Il7r^tm1.1(icre)Hrr/Il7r^+	involves: 129P2/OlaHsd * C57BL/6N	MGI:8221632

Genotype
MGI:8221632

cn1

• Allelic Composition: Rora^{tm1c(EUCOMM)Wtsi}/Rora^{tm1c(EUCOMM)Wtsi}; Il7r^{tm1.1(icre)Hrr}/Il7r⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

abnormal alveolar macrophage morphology ( J:345920 )

• after two consecutive intranasal doses of IL-25, mRNA expression of Rnase2a [ribonuclease, RNase A family, 2A (liver, eosinophil-derived neurotoxin), also known as Ear11] is nearly absent in sorted CD45+CD11c+SiglecF+F4/80+ alveolar macrophages, whereas Rnase2a/Ear11 expression is highly elevated in alveolar macrophages purified from IL-25-challenged Il7r^{tm1.1(icre)Hrr} heterozygous controls

impaired neutrophil recruitment ( J:345920 )

• after two consecutive intranasal doses of IL-25, total lung neutrophil number and neutrophil infiltration in the bronchoalveolar lavage (BAL) is significantly lower than in IL-25-challenged Il7r^{tm1.1(icre)Hrr} heterozygous controls

• complementation of IL-25 challenge with recombinant mouse (rm)Rnase2a fails to induce neutrophil recruitment in the lung

• however, unchallenged mice show a normal total lung neutrophil number/0.1g tissue relative to nave Il7r^{tm1.1(icre)Hrr} heterozygous controls

immune system

abnormal alveolar macrophage morphology ( J:345920 )

• after two consecutive intranasal doses of IL-25, mRNA expression of Rnase2a [ribonuclease, RNase A family, 2A (liver, eosinophil-derived neurotoxin), also known as Ear11] is nearly absent in sorted CD45+CD11c+SiglecF+F4/80+ alveolar macrophages, whereas Rnase2a/Ear11 expression is highly elevated in alveolar macrophages purified from IL-25-challenged Il7r^{tm1.1(icre)Hrr} heterozygous controls

impaired neutrophil recruitment ( J:345920 )

• after two consecutive intranasal doses of IL-25, total lung neutrophil number and neutrophil infiltration in the bronchoalveolar lavage (BAL) is significantly lower than in IL-25-challenged Il7r^{tm1.1(icre)Hrr} heterozygous controls

• complementation of IL-25 challenge with recombinant mouse (rm)Rnase2a fails to induce neutrophil recruitment in the lung

• however, unchallenged mice show a normal total lung neutrophil number/0.1g tissue relative to nave Il7r^{tm1.1(icre)Hrr} heterozygous controls

respiratory system

abnormal alveolar macrophage morphology ( J:345920 )

• after two consecutive intranasal doses of IL-25, mRNA expression of Rnase2a [ribonuclease, RNase A family, 2A (liver, eosinophil-derived neurotoxin), also known as Ear11] is nearly absent in sorted CD45+CD11c+SiglecF+F4/80+ alveolar macrophages, whereas Rnase2a/Ear11 expression is highly elevated in alveolar macrophages purified from IL-25-challenged Il7r^{tm1.1(icre)Hrr} heterozygous controls