Phenotypes associated with this allele

• Allele Symbol: Alg3^em1(IMPC)J
• Allele Name: endonuclease-mediated mutation 1, Jackson
• Allele ID: MGI:6194900
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Alg3^em1(IMPC)J/Alg3^em1(IMPC)J	C57BL/6NJ-Alg3^em1(IMPC)J/Mmjax	MGI:6492695

Genotype
MGI:6492695

hm1

• Allelic Composition: Alg3^em1(IMPC)J/Alg3^em1(IMPC)J
• Genetic Background: C57BL/6NJ-Alg3^em1(IMPC)J/Mmjax

Data Sources

IMPC Data for Alg3

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Alg3^em1(IMPC)J/Alg3^em1(IMPC)J (-/-) embryos are smaller by E9.5 and and show developmental delay by E10.5, with abnormal head shape, and cardiac defects.

cardiovascular system

abnormal heart morphology ( J:374598 )

• by E10.5, many homozygous embryos show cardiac defects

heart hemorrhage ( J:374598 )

• by E10.5, many homozygous embryos show abnormal blood pooling in the heart

intracranial hemorrhage ( J:374598 )

• by E10.5, many homozygous embryos show abnormal blood pooling in the head

craniofacial

abnormal head shape ( J:374598 )

• an abnormal head shape is observed by E10.5

embryo

embryonic growth retardation ( J:374598 )

• homozygous embryos are developmentally delayed by E10.5

• however, germ layer patterning is normal at E9.5

decreased embryo size ( J:374598 )

• homozygous embryos are normal at E8.5 but sometimes smaller at E9.5

• by E10.5, homozygous embryos are overtly smaller than control littermates

abnormal mesenchyme morphology ( J:374598 )

• at E10.5, the loose mesenchyme surrounding the neural tube, esp. in the anterior portion of the embryo, appears less dense

abnormal placenta labyrinth morphology ( J:374598 )

• at E10.5, placentas exhibit multiple labyrinth zone defects

abnormal trophoblast layer morphology ( J:374598 )

• at E10.5, the Tfeb+ labyrinth trophoblast layer is reduced in thickness but shows increased Tfeb (transcription factor EB) signal intensity, suggesting an increased density of Tfeb+ cells

increased spongiotrophoblast size ( J:374598 )

• at E10.5, placentas exhibit an expanded Tpbpa+ (trophoblast specific protein alpha-positive) spongiotrophoblast layer

abnormal placenta development ( J:374598 )

• although properly developed at E9.5, placentas show multiple defects at E10.5 which become more severe by E11.5

• at E10.5, chorioallantoic branching is severely impaired, as indicated by sparse Gcm1 (glial cells missing homolog 1) expression

growth/size/body

abnormal head shape ( J:374598 )

• an abnormal head shape is observed by E10.5

embryonic growth retardation ( J:374598 )

• homozygous embryos are developmentally delayed by E10.5

• however, germ layer patterning is normal at E9.5

decreased embryo size ( J:374598 )

• homozygous embryos are normal at E8.5 but sometimes smaller at E9.5

• by E10.5, homozygous embryos are overtly smaller than control littermates

limbs/digits/tail

short tail ( J:374598 )

• a minority of homozygous embryos show shortened tails by E9.5

abnormal tail position or orientation ( J:374598 )

• a minority of homozygous embryos show abnormal tail placement by E9.5

mortality/aging

prenatal lethality ( J:211773 )

IMPC - JAX

preweaning lethality, complete penetrance ( J:211773 )

IMPC - JAX

embryonic lethality during organogenesis, complete penetrance ( J:374598 )

• homozygous embryos are present in expected Mendelian ratios at E9.5 and E10.5 but absent at E12.5; all homozygous embryos are dying/undergoing resorption by E11.5

nervous system

intracranial hemorrhage ( J:374598 )

• by E10.5, many homozygous embryos show abnormal blood pooling in the head

abnormal forebrain morphology ( J:374598 )

• an abnormal forebrain shape is observed by E10.5