Phenotypes associated with this allele

• Allele Symbol: Gt(ROSA)26Sor^{tm1(CAG-Slc39a14*L438R)Wvh}
• Allele Name: targeted mutation 1, Wim Van Hul
• Allele ID: MGI:6159129
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm1(CAG-Slc39a14*L438R)Wvh/Gt(ROSA)26Sor^+ Tg(Ctsk-cre)1Rda/0	involves: C57BL/6	MGI:6159158
cn2	Gt(ROSA)26Sor^tm1(CAG-Slc39a14*L438R)Wvh/Gt(ROSA)26Sor^+ Tg(Runx2-icre)1Jtuc/0	involves: FVB/N	MGI:6159136

Genotype
MGI:6159158

cn1

• Allelic Composition: Gt(ROSA)26Sor^{tm1(CAG-Slc39a14*L438R)Wvh}/Gt(ROSA)26Sor⁺; Tg(Ctsk-cre)1Rda/0
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

skeleton

abnormal tibia morphology ( J:261326 )

♂ • tibia show a decrease in osteoclast-covered bone surface

♂ • however, osteoclast number and osteoblast-covered surface and number are unaltered

increased trabecular bone volume ( J:261326 )

♀ • trabecular bone volume is increased in lumbar spine of females

abnormal compact bone morphology ( J:261326 )

• males exhibit decreased cortical porosity in femurs while females exhibit increased cortical porosity in femurs

• however, trabecular bone is unaffected

increased bone trabecula number ( J:261326 )

♀ • trabecula number is increased in lumbar spine of females

decreased bone trabecular spacing ( J:261326 )

♀ • trabecular separation in lumbar spine of females is decreased

increased trabecular bone thickness ( J:261326 )

♀ • trabecular thickness is increased in lumbar spine of females

increased bone mineralization ( J:261326 )

♂ • tibial endocortical bone surface shows an increase in endosteal mineralizing surface

decreased bone ossification ( J:261326 )

♂ • trabecular bone surface of vertebral bodies shows a decrease in mineralizing surface and bone formation rate

♂ • however, biomechanical properties of femurs are similar to controls

limbs/digits/tail

abnormal tibia morphology ( J:261326 )

♂ • tibia show a decrease in osteoclast-covered bone surface

♂ • however, osteoclast number and osteoblast-covered surface and number are unaltered

craniofacial

craniofacial phenotype ( J:261326 )

♂N • mice exhibit normal calvarial bone thickness and porosity

Genotype
MGI:6159136

cn2

• Allelic Composition: Gt(ROSA)26Sor^{tm1(CAG-Slc39a14*L438R)Wvh}/Gt(ROSA)26Sor⁺; Tg(Runx2-icre)1Jtuc/0
• Genetic Background: involves: FVB/N

skeleton

abnormal osteoblast physiology ( J:261326 )

♂ • long bone osteoblasts exhibit an increased expression of inflammatory cytokines during osteoblast differentiation

increased osteoclast cell number ( J:261326 )

• osteoclast number is increased in lumbar vertebral bodies and tibiae and osteoclast-covered surface of lumbar vertebral bodies and tibiae is increased in males

• osteoclast surface per bone surface and osteoclast number per bone perimeter is increased in vertebral bodies of females

• however, no differences in osteoblast-covered surface or number are seen

decreased diameter of femur ( J:261326 )

• femurs exhibit a smaller midshaft diameter

decreased diameter of tibia ( J:261326 )

♀ • tibia show decreased midshaft diameter

decreased trabecular bone volume ( J:261326 )

• decrease in trabecular bone volume in femurs and lumbar spine of males

• decrease in trabecular bone volume in lumbar spine of females

abnormal compact bone morphology ( J:261326 )

♂ • femurs show decreased cortical porosity

increased femur compact bone thickness ( J:261326 )

• femurs and tibiae show an increase in cortical thickness

decreased bone trabecula number ( J:261326 )

• decrease in trabecula number in femurs and lumbar spine of males

• decrease in trabecula number in lumbar spine of females

increased bone trabecular spacing ( J:261326 )

♂ • increase in trabecular separation in femurs and lumbar spine

decreased trabecular bone connectivity density ( J:261326 )

♂ • decrease in trabecular bone connection density

decreased trabecular bone mass ( J:261326 )

• femurs show decreased trabecular mass

decreased bone mineralization ( J:261326 )

♂ • reduction in cortical mineralization of femurs

increased bone mineralization ( J:261326 )

• tibial endocortical bone surface shows an increase in endosteal mineralizing surface

increased bone ossification ( J:261326 )

♂ • tibial endocortical bone surface shows an increase in endosteal bone formation rate

♂ • however, no differences in periosteal or trabecular bone formation parameters

decreased femur stiffness ( J:261326 )

♂ • the elastic modulus and work to reach ultimate stress levels are lower in femurs, indicating that femurs tolerate higher stress levels and are more elastic/flexible

fragile skeleton ( J:261326 )

♂ • two mice exhibit fracture with callus in the tibia

♂ • femurs bear higher stress levels in the three-point bending tests but work-to-fracture is 42% lower

cellular

abnormal osteoblast physiology ( J:261326 )

♂ • long bone osteoblasts exhibit an increased expression of inflammatory cytokines during osteoblast differentiation

hematopoietic system

increased osteoclast cell number ( J:261326 )

• osteoclast number is increased in lumbar vertebral bodies and tibiae and osteoclast-covered surface of lumbar vertebral bodies and tibiae is increased in males

• osteoclast surface per bone surface and osteoclast number per bone perimeter is increased in vertebral bodies of females

• however, no differences in osteoblast-covered surface or number are seen

immune system

increased osteoclast cell number ( J:261326 )

• osteoclast number is increased in lumbar vertebral bodies and tibiae and osteoclast-covered surface of lumbar vertebral bodies and tibiae is increased in males

• osteoclast surface per bone surface and osteoclast number per bone perimeter is increased in vertebral bodies of females

• however, no differences in osteoblast-covered surface or number are seen

limbs/digits/tail

increased femur compact bone thickness ( J:261326 )

• femurs and tibiae show an increase in cortical thickness

decreased diameter of femur ( J:261326 )

• femurs exhibit a smaller midshaft diameter

decreased diameter of tibia ( J:261326 )

♀ • tibia show decreased midshaft diameter

craniofacial

craniofacial phenotype ( J:261326 )

N • mice exhibit normal calvarial bone thickness and porosity