Tg(Runx2-icre)1Jtuc
Transgene Detail

Summary

• Symbol: Tg(Runx2-icre)1Jtuc
• Name: transgene insertion 1, Jan Tuckermann
• MGI ID: MGI:4455015
• Synonyms: Runx2Cre, Runx2-Cre
• Transgene: Tg(Runx2-icre)1Jtuc Location: unknown
• Alliance: Tg(Runx2-icre)1Jtuc page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Runx2-icre)1Jtuc expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Runx2

• Mutation details: A BAC containing the Runx2 locus was modified through ET recombination to insert a codon-improved cre recombinase sequence and an ampicillin resistance gene flanked by two FRT sites at the translational start site of the distal promoter (P1) of the Runx2 gene. Subsequently in the recombinant BAC, the ampicillin resistance cassette was removed by arabinose-induced FLP recombination.The linearized construct was injected into the pronuclei of FVB/N oocytes. Three founder lines were established and one was chosen for experimental use based on its expression pattern. (J:160669)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Runx2 (species not specified) Summary of all recombinase alleles driven by Runx2. Comparative matrix view of recombinase activities

Expression

In Mice Carrying this Mutation:

5 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:160669 Rauch A, et al., Glucocorticoids suppress bone formation by attenuating osteoblast differentiation via the monomeric glucocorticoid receptor. Cell Metab. 2010 Jun 9;11(6):517-31
• All: 25 reference(s)