Phenotypes associated with this allele

• Allele Symbol: Mfsd6^tm1Ryosh
• Allele Name: targeted mutation 1, Ryotaro Yoshida
• Allele ID: MGI:6157951
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mfsd6^tm1Ryosh/Mfsd6^tm1Ryosh	B6.129S2-Mfsd6^tm1Ryosh	MGI:8210920

Genotype
MGI:8210920

hm1

• Allelic Composition: Mfsd6^tm1Ryosh/Mfsd6^tm1Ryosh
• Genetic Background: B6.129S2-Mfsd6^tm1Ryosh

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:260694 )

N • mice are viable and healthy and show no major changes in total cell number and composition of cell subsets (CD3+, CD4+, CD8+, B220+, Mac-1+, and Ly-6G+) in the thymus, spleen, mesenteric lymph node and bone marrow, indicating normal leukocyte development in lymphoid tissues

N • mice exhibit normal alloreactive CD3+ T-cell proliferation in mixed lymphocyte culture assays, as determined by the % of CFSE-low cells within the CD3+ T cell population, and a normal delayed-type hypersensitivity (DTH) response to 2,4-dinitrofluorobenzene (DNFB), as determined by ear thickness at 24 and 48 hr after challenge

N • when transgenic EL-4 lymphoma cells are injected intradermally into mice, 30%, 17%, and 82% of mice bearing D^d-, K^d-, and D^dK^d-EL-4 cells, respectively, reject the grafts on days 12 to 14 after transplantation, whereas wild-type EL-4 tumor cells are tolerated, indicating transgene number-dependent and MMR-independent rejection of D^d-, K^d-, and D^dK^d-EL-4 cells

N • D^dK^d EL-4 lymphoma cells injected intraperitoneally into mice are rejected by TCR_{alpha beta}+/CD8+ T cells, as seen in similarly treated wild-type mice

abnormal leukocyte physiology ( J:260694 )

• surprisingly, peripheral blood mononuclear cells (PBMCs) and monocytes show loss of mRNA expression of both MMR1 and ATP9B (a precursor of MMR1), resulting in the tolerance to skin grafts from D^d or D^dK^d transgenic mice

• no binding of phycoerythrin-labeled H-2D^d pentamers to PBMCs is observed, unlike PBMCs from wild-type C57BL/6 mice which bind H-2D^d molecules with a very low K^d value

increased length of allograft survival ( J:260694 )

• mice fail to reject skin grafts from D^d, K^d, or D^dK^d transgenic C57BL/6 (H-2D^bK^b) mice

• in contrast, skin grafts from the B10D2 (H-2I^d/minor H^b), BALB.B (H-2^b/minor H^d), and C3H/He (H-2^k/minor H^k) strains are rejected on days 12 to 14 after transplantation

hematopoietic system

abnormal leukocyte physiology ( J:260694 )

• surprisingly, peripheral blood mononuclear cells (PBMCs) and monocytes show loss of mRNA expression of both MMR1 and ATP9B (a precursor of MMR1), resulting in the tolerance to skin grafts from D^d or D^dK^d transgenic mice

• no binding of phycoerythrin-labeled H-2D^d pentamers to PBMCs is observed, unlike PBMCs from wild-type C57BL/6 mice which bind H-2D^d molecules with a very low K^d value

growth/size/body

growth/size/body region phenotype ( J:260694 )

N • mice exhibit normal body weight relative to wild-type controls

reproductive system

reproductive system phenotype ( J:260694 )

N • mice exhibit normal fertility