Mfsd6^tm1Ryosh
Targeted Allele Detail

Summary

• Symbol: Mfsd6^tm1Ryosh
• Name: major facilitator superfamily domain containing 6; targeted mutation 1, Ryotaro Yoshida
• MGI ID: MGI:6157951
• Synonyms: MMR2 KO
• Gene: Mfsd6 Location: Chr1:52695463-52766495 bp, - strand Genetic Position: Chr1, 26.99 cM
• Alliance: Mfsd6^tm1Ryosh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:260694
• Parent Cell Line: EGR-05 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1 of the Mfsd6 gene contains the initiation codon and the H-2K^d binding motif. A neomycin resistance gene replaced an approximately 1.6-kb fragment containing a part (345 bp) of exon 1. RT-PCR analysis demonstrated the absence of mRNA expression in peripheral blood mononuclear cells (PBMCs) and monocytes from homozygous mutant mice. Flow cytometry analysis revealed no binding of phycoerythrin-labeled H-2K^d pentamers to mutant PBMCs. (J:260694)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mfsd6 Mutation: 45 strains or lines available

References

• Original: J:260694 Tashiro-Yamaji J, et al., Macrophage MHC and T-cell receptors essential for rejection of allografted skin and lymphoma. Transplantation. 2013 Aug 15;96(3):251-7
• All: 1 reference(s)