Phenotypes associated with this allele

• Allele Symbol: Shisa7^tm1.2Sasp
• Allele Name: targeted mutation 1.2, Sabine Spijker
• Allele ID: MGI:6154949
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Shisa7^tm1.2Sasp/Shisa7^tm1.2Sasp	involves: C57BL/6J	MGI:6155007

Genotype
MGI:6155007

hm1

• Allelic Composition: Shisa7^tm1.2Sasp/Shisa7^tm1.2Sasp
• Genetic Background: involves: C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

nervous system phenotype ( J:255015 )

N • series resistance and resting membrane potential in CA1 pyramidal neuron synapses in acute hippocampal slices

N • evoked excitatory postsynaptic current (eEPSC) amplitudes in CA1 pyramidal neurons

N • input-output curves of AMPA-type glutamate receptor (AMPAR) evoked excitatory postsynaptic currents in CA1 pyramidal neurons

N • paired-pulse ratios at any tested frequency in CA1 pyramidal neurons

N • synaptic depression with stimulation train of 10 pulses at 5, 20 or 50 Hz in CA1 pyramidal neurons

short excitatory postsynaptic current decay time ( J:255015 )

• of AMPAR eEPSCs in CA1 pyramidal neurons

• NMDAR eEPSCs in CA1 pyramidal neurons

short excitatory postsynaptic current rise time ( J:255015 )

• NMDAR eEPSCs in CA1 pyramidal neurons

• of AMPAR eEPSCs in CA1 pyramidal neurons

reduced AMPA-mediated synaptic currents ( J:255015 )

• faster deactivation kinetics of AMPA-type glutamate receptors (AMPARs) in CA1 pyramidal neurons in acute hippocampal slices

reduced long-term potentiation ( J:255015 )

• decrease in maintenance phase of hippocampal LTP in CA1 pyramidal neurons

• synaptic potentiation between 20-25 min. and 25-30 min. after stimulation in CA1 pyramidal neurons

• reduced LTP initiation during first 5 min. after theta burst stimulation in CA1 pyramidal neurons MP:0009454 impaired contextual conditioning behavior

abnormal miniature excitatory postsynaptic currents ( J:255015 )

• faster deactivation kinetics of AMPA-type glutamate receptors in CA1 pyramidal neurons in acute hippocampal slices

• rise time, amplitude and frequency of AMPAR currents in CA1 pyramidal neurons in acute hippocampal slices

behavior/neurological

behavior/neurological phenotype ( J:255015 )

N • behavior in open field test, elevated plus maze and dark-light box tests

N • baseline locomotor activity and ability to sense electric shock in conditioned fear test

N • freezing behavior in auditory fear conditioning test (audio signal + electric shock)

impaired contextual conditioning behavior ( J:255015 )

• deficit in contextual fear memory as seen by reduced freezing behavior 2 h and 24 h after conditioning by electric shock

fast extinction of fear memory ( J:255015 )

• deficit in contextual fear memory as seen by reduced freezing behavior 2 h and 24 h after conditioning by electric shock