Shisa7^tm1.2Sasp
Targeted Allele Detail

Summary

• Symbol: Shisa7^tm1.2Sasp
• Name: shisa family member 7; targeted mutation 1.2, Sabine Spijker
• MGI ID: MGI:6154949
• Synonyms: Shisa7 KO, Shisa7^{tm1b(Caliper)CNCR-VUA}
• Gene: Shisa7 Location: Chr7:4828551-4847695 bp, - strand Genetic Position: Chr7, 2.79 cM
• Alliance: Shisa7^tm1.2Sasp page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:255015
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted into intron 1 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 3. The neo cassette was removed through subsequent flp-mediated recombination and exons 2 and 3 were deleted through subsequent cre-mediated recombination. Exon 2 contains the ATG translation start site. Immunoblots of hippocampal crude synaptic membrane fractions confirmed the lack of protein expression from this allele. (J:255015)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Shisa7 Mutation: 12 strains or lines available

References

• Original: J:255015 Schmitz LJM, et al., The AMPA receptor-associated protein Shisa7 regulates hippocampal synaptic function and contextual memory. Elife. 2017 Dec 4;6:e24192
• All: 1 reference(s)