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Allele Symbol Allele Name Allele ID |
Bcas2tm1.1Lil targeted mutation 1.1, Lei Li MGI:5909974 |
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| Summary |
3 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• aberrant alternative splicing (AS) events are detected in numerous oogenesis genes, such as Pabpc1l and Dazl
• protein level of other PRP19 complex core components (CDC5L and PRP19) is severely reduced in GV oocytes as early as P5; however, their mRNA levels remain normal in oocytes from P10 females
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• meiosis II oocytes exhibit a large amount of dark cytoplasmic granules as well as disorganized and larger spindles than control oocytes
• the diameter of germinal vesicle (GV) oocytes without zona pellucida is significantly smaller (72.4 um) than in control GV oocytes (85.8 um)
• Nile red staining showed increased lipid droplet aggregation, esp. in the peripheral cytoplasm of GV oocytes
• Mitotracker Red CMXROS staining showed absence of mitochondria around the nucleus and reduced fluorescence intensity in GV oocytes, suggesting poor oocyte quality
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• 94% of GV oocytes are found in the nonsurrounded nucleolus (NSN) stage, whereas 80% of control oocytes are in the surrounded nucleolus (SN) stage, suggesting defects in nucleus maturation
• upon culture of GV oocytes, the rate of germinal vesicle breakdown is significantly lower (30%) than in control oocytes (90%)
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• the number of primary follicles is normal at P10, P16 and P23 but significantly increased at 2 months of age
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• the number of secondary follicles is normal at P10, P16 and P23 but significantly increased at 2 months of age
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• the number of antral follicles is normal at P10 but significantly decreased at P16, at P23 and at 2 months of age
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• follicular development is arrested: primary follicles and secondary follicles fail to develop into antral follicles
• however, the number of primordial follicles and total number of all stages of follicles is relatively normal from P10 to 2 months of age, indicating that establishment and recruitment of the primordial follicle pool is largely unaffected
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• ovaries begin to decrease in size at P16 and are obviously smaller than control ovaries after P23
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• following superovulation induction, the number of ovulated oocytes retrieved is less than 50% of that in control females
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• female mice grow normally into adulthood but are infertile
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• when adult females are mated with normal fertile males, no 2-cell embryos are obtained from fallopian tubes on E1.5
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• aberrant alternative splicing (AS) events are detected in numerous oogenesis genes, such as Pabpc1l and Dazl
• protein level of other PRP19 complex core components (CDC5L and PRP19) is severely reduced in GV oocytes as early as P5; however, their mRNA levels remain normal in oocytes from P10 females
|
|
|
• meiosis II oocytes exhibit a large amount of dark cytoplasmic granules as well as disorganized and larger spindles than control oocytes
• the diameter of germinal vesicle (GV) oocytes without zona pellucida is significantly smaller (72.4 um) than in control GV oocytes (85.8 um)
• Nile red staining showed increased lipid droplet aggregation, esp. in the peripheral cytoplasm of GV oocytes
• Mitotracker Red CMXROS staining showed absence of mitochondria around the nucleus and reduced fluorescence intensity in GV oocytes, suggesting poor oocyte quality
|
|
|
• 94% of GV oocytes are found in the nonsurrounded nucleolus (NSN) stage, whereas 80% of control oocytes are in the surrounded nucleolus (SN) stage, suggesting defects in nucleus maturation
• upon culture of GV oocytes, the rate of germinal vesicle breakdown is significantly lower (30%) than in control oocytes (90%)
|
|
|
• the number of primary follicles is normal at P10, P16 and P23 but significantly increased at 2 months of age
|
|
|
• the number of secondary follicles is normal at P10, P16 and P23 but significantly increased at 2 months of age
|
|
|
• the number of antral follicles is normal at P10 but significantly decreased at P16, at P23 and at 2 months of age
|
|
|
• follicular development is arrested: primary follicles and secondary follicles fail to develop into antral follicles
• however, the number of primordial follicles and total number of all stages of follicles is relatively normal from P10 to 2 months of age, indicating that establishment and recruitment of the primordial follicle pool is largely unaffected
|
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• ovaries begin to decrease in size at P16 and are obviously smaller than control ovaries after P23
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/07/2024 MGI 6.23 |
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