About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Orai2tm1.1(KOMP)Vlcg
targeted mutation 1.1, Velocigene
MGI:5908178
Summary 2 genotypes


Genotype
MGI:5908388
hm1
Allelic
Composition		 Orai2tm1.1(KOMP)Vlcg/Orai2tm1.1(KOMP)Vlcg
Genetic
Background		 B6.Cg-Orai2tm1.1(KOMP)Vlcg
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Orai2tm1.1(KOMP)Vlcg mutation (0 available); any Orai2 mutation (14 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal T cell physiology ( J:241365 )
• SOCE (store-operated Ca2+ entry) increased in naive CD4+ and CD8+ T cells after induction by depletion of ER Ca2+ stores with thapsigargin
abnormal macrophage physiology ( J:241365 )
• CRAC (Ca2+ release-activated Ca2+) currents significantly larger

immune system
abnormal T cell physiology ( J:241365 )
• SOCE (store-operated Ca2+ entry) increased in naive CD4+ and CD8+ T cells after induction by depletion of ER Ca2+ stores with thapsigargin
abnormal macrophage physiology ( J:241365 )
• CRAC (Ca2+ release-activated Ca2+) currents significantly larger




Genotype
MGI:5908389
cn2
Allelic
Composition		 Orai1tm1.1Hjm/Orai1tm1.1Hjm
Orai2tm1.1(KOMP)Vlcg/Orai2tm1.1(KOMP)Vlcg
Tg(Cd4-cre)1Cwi/0
Genetic
Background		 B6.Cg-Tg(Cd4-cre)1Cwi Orai1tm1.1Hjm Orai2tm1.1(KOMP)Vlcg
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Orai1tm1.1Hjm mutation (0 available); any Orai1 mutation (21 available)
Orai2tm1.1(KOMP)Vlcg mutation (0 available); any Orai2 mutation (14 available)
Tg(Cd4-cre)1Cwi mutation (10 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
hematopoietic system phenotype ( J:241365 )
N
• frequencies of T cells
spleen hyperplasia ( J:241365 )
• elevated numbers of immune cell populations at age >3 months
• elevated numbers of CD4+ and CD8+ T cells with CD44hiCD62Lhi memory and/or CD44hiCD62Llo effector phenotypes
decreased germinal center B cell number ( J:241365 )
• CD19+ CD38- GL.7+ in spleen after lymphocytic choriomeningitis virus (LCMV) infection
decreased regulatory T cell number ( J:241365 )
• decreased Foxp3+ cells in thymus, spleen and lymph nodes
decreased single-positive T cell number ( J:241365 )
• severely compromised proliferation of CD4+ cells
abnormal CD4-positive helper T cell morphology ( J:241365 )
• reduced number in spleen after lymphocytic choriomeningitis virus (LCMV) infection
decreased IgG level ( J:241365 )
• after lymphocytic choriomeningitis virus (LCMV) infection
abnormal T cell physiology ( J:241365 )
• SOCE (store-operated Ca2+ entry) completely abolished in naive CD4+ and CD8+ T cells after induction by depletion of ER Ca2+ stores with thapsigargin

immune system
immune system phenotype ( J:241365 )
N
• IgM level after lymphocytic choriomeningitis virus (LCMV) infection
spleen hyperplasia ( J:241365 )
• elevated numbers of immune cell populations at age >3 months
• elevated numbers of CD4+ and CD8+ T cells with CD44hiCD62Lhi memory and/or CD44hiCD62Llo effector phenotypes
decreased germinal center B cell number ( J:241365 )
• CD19+ CD38- GL.7+ in spleen after lymphocytic choriomeningitis virus (LCMV) infection
decreased regulatory T cell number ( J:241365 )
• decreased Foxp3+ cells in thymus, spleen and lymph nodes
decreased single-positive T cell number ( J:241365 )
• severely compromised proliferation of CD4+ cells
abnormal CD4-positive helper T cell morphology ( J:241365 )
• reduced number in spleen after lymphocytic choriomeningitis virus (LCMV) infection
decreased IgG level ( J:241365 )
• after lymphocytic choriomeningitis virus (LCMV) infection
abnormal T cell physiology ( J:241365 )
• SOCE (store-operated Ca2+ entry) completely abolished in naive CD4+ and CD8+ T cells after induction by depletion of ER Ca2+ stores with thapsigargin
lymph node hyperplasia ( J:241365 )
• elevated numbers of immune cell populations at age >3 months

growth/size/body
spleen hyperplasia ( J:241365 )
• elevated numbers of immune cell populations at age >3 months
• elevated numbers of CD4+ and CD8+ T cells with CD44hiCD62Lhi memory and/or CD44hiCD62Llo effector phenotypes




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
05/07/2024
MGI 6.23 		The Jackson Laboratory