Orai2^{tm1.1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Orai2^{tm1.1(KOMP)Vlcg}
• Name: ORAI calcium release-activated calcium modulator 2; targeted mutation 1.1, Velocigene
• MGI ID: MGI:5908178
• Synonyms: Orai2^LacZ
• Gene: Orai2 Location: Chr5:136176313-136199567 bp, - strand Genetic Position: Chr5, 75.84 cM
• Alliance: Orai2^{tm1.1(KOMP)Vlcg} page
• IMPC: Orai2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241365
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 11345bp between positions 136626234-136637578 of Chromosome 5 (Genome Build37), deleting virtually the entire coding region (located on exons 2 and 3). The cassette contains a lacZ reporter gene cassette and a loxP site flanked neomycin resistance gene cassette. The neo gene was removed through subsequent cre-mediated recombination. The absence of transcription and translation from this allele was confirmed by qRT-PCR and immunoblots. (J:241365)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Orai2 Mutation: 14 strains or lines available

References

• Original: J:241365 Vaeth M, et al., ORAI2 modulates store-operated calcium entry and T cell-mediated immunity. Nat Commun. 2017 Mar 15;8:14714
• All: 2 reference(s)