Analysis Tools
liver/biliary system
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• mice fed a high-fat diet show decreased liver glycogen stores, as shown by periodic acid Schiff staining
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• mice fed a high-fat diet show increased hepatic triglyceride levels
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• mice fed a high-fat diet show significant hepatic lipid accumulation, as determined by Oil Red O staining and hepatic fat measured as % of total liver weight by Echo-MRI
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homeostasis/metabolism
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• mice fed a high-fat diet show significantly reduced phosphorylation of ACC and AMPK as well as reduced PPARalpha expression and activity in the liver, indicating reduced burning of fat through beta-oxidation
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• on a high fat diet, mice exhibit enhanced fasting hyperglycemia relative to control mice
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• on a high fat diet, mice exhibit enhanced fasting hyperinsulinemia relative to control mice
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• mice fed a high-fat diet show higher blood glucose levels than control mice at 30, 60, and 90 min after i.p. glucose injection during glucose tolerance testing (IPGTT)
• no differences are noted during insulin tolerance testing, suggesting decreased hepatic insulin sensitivity
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• mice fed a high-fat diet show decreased liver glycogen stores, as shown by periodic acid Schiff staining
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• on a high fat diet, mice exhibit reduced insulin signaling and hepatic insulin resistance
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• hepatic steatosis is associated with enhanced hepatic expression of proteins and signaling pathways involved in fatty acid synthesis (e.g. reduced levels of phosphorylated AMPK, increased fatty acid synthase expression, and reduced levels of phosphorylated acetyl-CoA carboxylase (ACC) indicating enhanced ACC activity)
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• mice fed a high-fat diet show increased hepatic triglyceride levels
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cellular
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• mice fed a high-fat diet show significantly reduced phosphorylation of ACC and AMPK as well as reduced PPARalpha expression and activity in the liver, indicating reduced burning of fat through beta-oxidation
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