Phenotypes associated with this allele

• Allele Symbol: Gpr4^tm1Mglg
• Allele Name: targeted mutation 1, Marie-Gabrielle Ludwig
• Allele ID: MGI:5776512
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gpr4^tm1Mglg/Gpr4^tm1Mglg	either: BALB/c-Gpr4^tm1Mglg or B6.C-Gpr4^tm1Mglg	MGI:5776699
hm2	Gpr4^tm1Mglg/Gpr4^tm1Mglg	either: (involves: BALB/c) or (involves: BALB/c * C57BL/6J)	MGI:5776703
cx3	Gpr4^tm1Mglg/Gpr4^tm1Mglg Tg(Phox2b-EGFP)JX99Gsat/0	either: (involves: BALB/c * FVB/NTac) or (involves: BALB/c * C57BL/6J * FVB/NTac)	MGI:5776707

Genotype
MGI:5776699

hm1

• Allelic Composition: Gpr4^tm1Mglg/Gpr4^tm1Mglg
• Genetic Background: either: BALB/c-Gpr4^tm1Mglg or B6.C-Gpr4^tm1Mglg

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

neoplasm

abnormal tumor vascularization ( J:232324 )

• in implanted 4T1 breast tumor cells or syngenic CT26 colon tumor cells

decreased tumor growth/size ( J:232324 )

• implanted 4T1 breast tumor cells or syngenic CT26 colon tumor cells exhibit reduced growth with decreased tumor cell proliferation and reduced vascularity compared with cells implanted in wild-type mice

cardiovascular system

abnormal tumor vascularization ( J:232324 )

• in implanted 4T1 breast tumor cells or syngenic CT26 colon tumor cells

decreased angiogenesis ( J:232324 )

• in response to VEGF

• however, response to bFGF is normal

Genotype
MGI:5776703

hm2

• Allelic Composition: Gpr4^tm1Mglg/Gpr4^tm1Mglg
• Genetic Background: either: (involves: BALB/c) or (involves: BALB/c * C57BL/6J)

homeostasis/metabolism

increased urine creatinine level ( J:224758 )

• under baseline conditions and 24 hrs after NH4Cl-feeding

increased urine ammonia level ( J:224758 )

• relative to creatinine levels 24 hrs after NH4Cl-feeding

hypercapnia ( J:224758 )

• under baseline conditions

acidosis ( J:224758 )

• under baseline conditions

renal tubular acidosis ( J:224758 )

• mild under baseline conditions

decreased urine calcium level ( J:224758 )

• relative to creatinine levels 24 hrs after NH4Cl-feeding

increased urine osmolality ( J:224758 )

• under baseline conditions and 24 hrs after NH4Cl-feeding

increased urine phosphate level ( J:224758 )

• relative to creatinine levels under baseline conditions but not 24 hrs after NH4Cl-feeding

respiratory system

apnea ( J:224758 )

• 6 times higher than in control mice

• however, re-expression of the endogenous protein reduces the number of apneic events

decreased pulmonary ventilation ( J:224758 )

• over a range of CO2 concentrations, even under high O2 conditions

• however, ventilatory response to hypoxia is normal

renal/urinary system

increased urine creatinine level ( J:224758 )

• under baseline conditions and 24 hrs after NH4Cl-feeding

increased urine ammonia level ( J:224758 )

• relative to creatinine levels 24 hrs after NH4Cl-feeding

renal tubular acidosis ( J:224758 )

• mild under baseline conditions

decreased urine calcium level ( J:224758 )

• relative to creatinine levels 24 hrs after NH4Cl-feeding

increased urine osmolality ( J:224758 )

• under baseline conditions and 24 hrs after NH4Cl-feeding

increased urine phosphate level ( J:224758 )

• relative to creatinine levels under baseline conditions but not 24 hrs after NH4Cl-feeding

oliguria ( J:224758 )

• under baseline conditions and 24 hrs after NH4Cl-feeding

cardiovascular system

cardiovascular system phenotype ( J:224758 )

N • mice do not exhibit any vascular abnormalities

behavior/neurological

behavior/neurological phenotype ( J:224758 )

N • mice exhibit normal spontaneous activity and exploratory behavior

Genotype
MGI:5776707

cx3

• Allelic Composition: Gpr4^tm1Mglg/Gpr4^tm1Mglg; Tg(Phox2b-EGFP)JX99Gsat/0
• Genetic Background: either: (involves: BALB/c * FVB/NTac) or (involves: BALB/c * C57BL/6J * FVB/NTac)

nervous system

abnormal nerve fiber response ( J:224758 )

• many retrotrapezoid nucleus (RTN) neurons are pH-insensitive and those that remain pH-sensitive exhibit lower firing rates across a pH range compared with control cells

• under voltage clamp, the pH-dependent background potassium current is absent unlike in control cells

• when dialyzed with GTP-gamma-S, RTN neurons exhibit diminished membrane potential with repeated pH changes unlike wild-type cells