Phenotypes associated with this allele

• Allele Symbol: Scyl2^tm1.1Spel
• Allele Name: targeted mutation 1.1, Stephane Pelletier
• Allele ID: MGI:5752500
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Scyl2^tm1.1Spel/Scyl2^tm1.1Spel	involves: 129S6/SvEvTac * C57BL/6	MGI:5752572
cn2	Bax^tm2Sjk/Bax^tm2Sjk Scyl2^tm1.1Spel/Scyl2^tm1.1Spel Tg(Nes-cre)1Kln/0	involves: 129S6/SvEvTac * 129X1/SvJ * C57BL/6 * SJL	MGI:5752574
cn3	Scyl2^tm1.1Spel/Scyl2^tm1.1Spel Tg(Nes-cre)1Kln/0	involves: 129S6/SvEvTac * C57BL/6 * SJL	MGI:5752573

Genotype
MGI:5752572

hm1

• Allelic Composition: Scyl2^tm1.1Spel/Scyl2^tm1.1Spel
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:225808 )

• mice are viable, fertile, and exhibit no overt abnormalities

Genotype
MGI:5752574

cn2

• Allelic Composition: Bax^tm2Sjk/Bax^tm2Sjk; Scyl2^tm1.1Spel/Scyl2^tm1.1Spel; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: 129S6/SvEvTac * 129X1/SvJ * C57BL/6 * SJL

mortality/aging

preweaning lethality, incomplete penetrance ( J:225808 )

• fewer than expected mice are present at weaning

growth/size/body

decreased body size ( J:225808 )

behavior/neurological

behavior/neurological phenotype ( J:225808 )

N • mice do not exhibit limb clasping as in Scyl2^tm1.1Spel/Scyl2^tm1.1Spel Tg(Nes-cre)1Kln mice

nervous system

nervous system phenotype ( J:225808 )

N • mice exhibit nor brain abnormalities unlike in Scyl2^tm1.1Spel/Scyl2^tm1.1Spel Tg(Nes-cre)1Kln mice

Genotype
MGI:5752573

cn3

• Allelic Composition: Scyl2^tm1.1Spel/Scyl2^tm1.1Spel; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * SJL

mortality/aging

neonatal lethality, incomplete penetrance ( J:225808 )

• just over half of mice die within 24 h of birth due to malnutrition

reproductive system

priapism ( J:225808 )

• in mice that survive beyond the neonatal period

nervous system

astrocytosis ( J:225808 )

• in the CA3 region

microgliosis ( J:225808 )

• in the CA3 region

increased neuron apoptosis ( J:225808 )

• of pyramidal CA3 neurons at P4.5, P6.5 and P8.5

• in the granular layer of the cerebellum of P6.5

decreased brain size ( J:225808 )

decreased dentate gyrus size ( J:225808 )

• decreased cellularity by P14.5 and P21.5

decreased hippocampus pyramidal cell number ( J:225808 )

• near complete absence of pyramidal CA3 neurons beginning between P6.5 and P8.5

hippocampal neuron degeneration ( J:225808 )

• near complete absence of pyramidal CA3 neurons

• however, CA3 neuron loss is prevented by treatment with MK-801, an irreversible NMDA receptor antagonist

small hippocampus ( J:225808 )

• however, the CA1 region is normal in cellularity

abnormal excitatory postsynaptic potential ( J:225808 )

• decreased field excitatory postsynaptic potential in hippocampal slices

behavior/neurological

abnormal behavior ( J:225808 )

• sensory-motor deficits and abnormal behaviors in mice that survive beyond the neonatal period

abnormal depression-related behavior ( J:225808 )

• depression-like behavior (lower latency to immobility in a tail suspension test) in mice that survive beyond the neonatal period

• however, time spent immobile is normal

limb grasping ( J:225808 )

• in mice that survive beyond the neonatal period

tremors ( J:225808 )

• in mice that survive beyond the neonatal period

decreased grip strength ( J:225808 )

• detected using an inverted cage grid in mice that survive beyond the neonatal period

growth/size/body

decreased body weight ( J:225808 )

• detected at 2 weeks in mice that survive beyond the neonatal period

postnatal growth retardation ( J:225808 )

• detected at 2 weeks in mice that survive beyond the neonatal period

hematopoietic system

microgliosis ( J:225808 )

• in the CA3 region

cellular

astrocytosis ( J:225808 )

• in the CA3 region

microgliosis ( J:225808 )

• in the CA3 region

increased neuron apoptosis ( J:225808 )

• of pyramidal CA3 neurons at P4.5, P6.5 and P8.5

• in the granular layer of the cerebellum of P6.5

immune system

microgliosis ( J:225808 )

• in the CA3 region