Phenotypes associated with this allele

• Allele Symbol: Gt(ROSA)26Sor^{tm2(CAG-Lancl1)Pfw}
• Allele Name: targeted mutation 2, Paul Worley
• Allele ID: MGI:5646274
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm2(CAG-Lancl1)Pfw/Gt(ROSA)26Sor^+ Tg(Ddx4-cre)1Dcas/0	involves: C57BL/6 * FVB	MGI:7294209
cn2	Gt(ROSA)26Sor^tm2(CAG-Lancl1)Pfw/Gt(ROSA)26Sor^tm2(CAG-Lancl1)Pfw Tg(Nes-cre)1Kln/0	involves: C57BL/6 * SJL	MGI:5646277

Genotype
MGI:7294209

cn1

• Allelic Composition: Gt(ROSA)26Sor^{tm2(CAG-Lancl1)Pfw}/Gt(ROSA)26Sor⁺; Tg(Ddx4-cre)1Dcas/0
• Genetic Background: involves: C57BL/6 * FVB

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

abnormal male germ cell apoptosis ( J:324139 )

♂ • males fed a high-fat diet (HFD) for 14 weeks (to induce obesity) show a significantly lower testicular expression of BAX and cleaved-caspase-3 protein than HFD-fed wild-type males

abnormal sperm progressive motility ( J:324139 )

♂ • males fed a HFD for 14 weeks show a significantly higher sperm forward motility (%) than HFD-fed wild-type males

increased sperm motility ( J:324139 )

♂ • males fed a HFD for 14 weeks show a significantly higher sperm motility (%) than HFD-fed wild-type males

increased sperm number ( J:324139 )

♂ • males fed a HFD for 14 weeks show a significantly higher caudal epididymal sperm concentration than HFD-fed wild-type males

abnormal spermatogenesis ( J:324139 )

♂ • males fed a HFD for 14 weeks show a less distorted arrangement of germ cells in the seminiferous tubules, a better Johnsen's score, and a significantly lower teratozoospermic ratio than HFD-fed wild-type males, indicating protection from HFD/obesity-induced spermatogenic defects

cellular

decreased cellular sensitivity to oxidative stress ( J:324139 )

♂ • males fed a HFD for 14 weeks show a significantly lower number of gammaH2AX+ germ cells per seminiferous tubule than HFD-fed wild-type males, indicating protection from HFD-induced oxidative damage

abnormal male germ cell apoptosis ( J:324139 )

♂ • males fed a high-fat diet (HFD) for 14 weeks (to induce obesity) show a significantly lower testicular expression of BAX and cleaved-caspase-3 protein than HFD-fed wild-type males

abnormal sperm progressive motility ( J:324139 )

♂ • males fed a HFD for 14 weeks show a significantly higher sperm forward motility (%) than HFD-fed wild-type males

increased sperm motility ( J:324139 )

♂ • males fed a HFD for 14 weeks show a significantly higher sperm motility (%) than HFD-fed wild-type males

abnormal redox activity ( J:324139 )

♂ • males fed a HFD for 14 weeks show improved testicular redox imbalance with a significantly higher NADPH/NADP ratio and significantly lower malondialdehyde (MDA) and protein carbonyls in the testes than HFD-fed wild-type males, indicating protection from HFD-induced oxidative stress

Genotype
MGI:5646277

cn2

• Allelic Composition: Gt(ROSA)26Sor^{tm2(CAG-Lancl1)Pfw}/Gt(ROSA)26Sor^{tm2(CAG-Lancl1)Pfw}; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: C57BL/6 * SJL

nervous system

decreased susceptibility to neuronal excitotoxicity ( J:214805 )

• cortical neurons treated with oxidative stress inducing agents (H2O2) show reduced cell death compared to wild-type controls

homeostasis/metabolism

decreased susceptibility to neuronal excitotoxicity ( J:214805 )

• cortical neurons treated with oxidative stress inducing agents (H2O2) show reduced cell death compared to wild-type controls

cellular

decreased susceptibility to neuronal excitotoxicity ( J:214805 )

• cortical neurons treated with oxidative stress inducing agents (H2O2) show reduced cell death compared to wild-type controls