Analysis Tools
normal phenotype
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• floxed mice show no differences in collagen V expression, gross morphology or cornea ultrastructure relative to wild-type controls
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Allele Symbol Allele Name Allele ID |
Col5a1tm2.1Rjw targeted mutation 2.1, Richard J Wenstrup MGI:5644153 |
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| Summary |
2 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• floxed mice show no differences in collagen V expression, gross morphology or cornea ultrastructure relative to wild-type controls
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• adult mice display abnormal fibril structure and stromal disorganization
• larger and less uniform collagen fibrils are seen throughout the cornea stroma, with disrupted fibril packing
• a significant decrease in fibril density and inter-fibril space is noted in both anterior and posterior regions of the stroma
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• adult mice exhibit a 14% decrease in corneal stroma thickness relative to wild-type controls
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• in the anterior stroma, the mean stromal fibril diameter increased to 42.5+/-12.8 nm versus 25.3+/-3.4 nm in control corneas
• the range of anterior stroma fibril diameters is 8-95 nm versus 14-42 nm in control corneas
• a 58% decrease in the number of fibrils per unit area is noted in the anterior stroma
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• in the posterior stroma, the mean stromal fibril diameter increased to 34.7+/-6.7 nm versus 27.5+/-3.3 nm in control corneas
• the range of posterior stroma fibril diameters is 12-61 nm versus 14-39 nm in control corneas
• a 36% decrease in the number of fibrils per unit area is noted in the posterior stroma
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• at P30 and P90, mice exhibit a heterogeneous collagen fibril population containing fibrils with very large diameters
• structurally abnormal fibrils with irregular profiles are commonly observed
• the fibril phenotype shows an anterior-posterior difference, with the anterior stroma containing larger and more heterogeneous fibrils than the posterior stroma
• a significant decrease in fibril density is noted in both anterior and posterior regions of the stroma
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• adult mice exhibit conspicuous corneal stromal opacity
• at P60, backscattered light is observed throughout the corneal stroma, with more haze in the anterior stroma
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• mice exhibit a 3.5-fold increase in the average light scattering per micrometer thickness of the corneal stroma relative to wild-type controls
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| N |
• mice are viable and fertile with no significant differences in body weight at P10 or P30 relative to wild-type controls
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 05/07/2024 MGI 6.23 |
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