Phenotypes associated with this allele
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Col1a1tm2(tetO-IDH2*R140Q)Ppp mutation
(1 available);
any
Col1a1 mutation
(161 available)
Flt3tm1.1Dosm mutation
(0 available);
any
Flt3 mutation
(83 available)
Gt(ROSA)26Sortm1(rtTA*M2)Jae mutation
(30 available);
any
Gt(ROSA)26Sor mutation
(944 available)
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neoplasm
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• mice treated with doxycycline develop acute leukemias
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mortality/aging
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• reduced overall survival of doxycycline treated mice to 229 days vs 352 days for Flt3tm1.1Dosm controls
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Col1a1tm2(tetO-IDH2*R140Q)Ppp mutation
(1 available);
any
Col1a1 mutation
(161 available)
Gt(ROSA)26Sortm1(rtTA*M2)Jae mutation
(30 available);
any
Gt(ROSA)26Sor mutation
(944 available)
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hematopoietic system
N |
• modified IDH2 expression induced by doxycycline in chow starting around 3 weeks
• peripheral blood cell counts, number of hematopoietic stem cells, myeloid progenitors, mature B and T lymphocytes, and myeloid cells all normal when examined 8 weeks after induction
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• KSL cells collected and plated out 8 weeks after induction display severely reduced erythroid colony formation but myeloid colony numbers are normal
• differentiation blockade is removed when doxycycline is removed from the culture medium
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• by 7 months KSL cells are increased in bone marrow relative to controls
• long-term hematopoietic stem cells also tend to expand in number
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• about 1.3X larger than control spleens
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• significant extramedullary hematopoiesis
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neoplasm
N |
• no mice on doxycycline treatment for 1 year develop leukemia
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• infection of KSL cells with retrovirus containing HoxA9 and Meis1 and then injected into sub-lethally irradiated mice results in a pre-leukemic cell expansion
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cellular
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• KSL cells collected and plated out 8 weeks after induction display severely reduced erythroid colony formation but myeloid colony numbers are normal
• differentiation blockade is removed when doxycycline is removed from the culture medium
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• by 7 months KSL cells are increased in bone marrow relative to controls
• long-term hematopoietic stem cells also tend to expand in number
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immune system
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• about 1.3X larger than control spleens
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growth/size/body
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• about 1.3X larger than control spleens
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