Phenotypes associated with this allele

• Allele Symbol: Jagn1^{tm1c(EUCOMM)Hmgu}
• Allele Name: targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• Allele ID: MGI:5604904
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Jagn1^tm1c(EUCOMM)Hmgu/Jagn1^tm1c(EUCOMM)Hmgu Commd10^Tg(Vav1-icre)A2Kio/Commd10^+	involves: C57BL/6N * C57BL/10 * CBA/Ca	MGI:5604905

Genotype
MGI:5604905

cn1

• Allelic Composition: Jagn1^{tm1c(EUCOMM)Hmgu}/Jagn1^{tm1c(EUCOMM)Hmgu}; Commd10^{Tg(Vav1-icre)A2Kio}/Commd10⁺
• Genetic Background: involves: C57BL/6N * C57BL/10 * CBA/Ca

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal neutrophil morphology ( J:214828 )

• neutrophils show reduced numbers of primary and secondary granules

• neutrophils show alterations in the glycosylation of proteins involved in cell adhesion and cytotoxicity

• however, mice exhibit normal numbers of neutrophils in the bone marrow, secondary lymphoid organs and blood

abnormal neutrophil physiology ( J:214828 )

• neutrophils, but not macrophages and dendritic cells, exhibit impaired killing of Candida albicans

• neutrophils show decreased ability to release myeloperoxidase when challenged in vitro with Candida albicans

impaired neutrophil chemotaxis ( J:214828 )

• in vitro assays show a migratory defect in neutrophils in response to the chemoattractant N-formyl-methionine-leucine-phenylalanine (fMLP)

impaired neutrophil recruitment ( J:214828 )

• recruitment of neutrophils to the peritoneal cavity following Candida albicans injection into the peritoneal cavity is reduced and mice show decreased recruitment of neutrophils to the kidney and lung 24 hours following systemic infection with Candida albicans, indicating that neutrophils cannot effectively migrate to the site of infection

increased susceptibility to fungal infection ( J:214828 )

• mice are unable to mount an efficient neutrophil-dependent immune response to the human fungal pathogen Candida albicans, showing increased weight loss, massively increased fungal burdens, severe multifocal tubulointerstitial nephritis, splenitis with expanded white pulp, diffuse inflammatory infiltrations into the liver and lungs, and decreased TNF-alpha levels in the serum and intraperitonal lavage and succumb earlier to Candida albicans infection

• treatment with granulocyte/macrophage colony-stimulating factor (GM-CSF) protects mutants from weight loss, rescues the neutrophil recruitment defects and increases survival after Candida albicans challenge

cellular

impaired neutrophil chemotaxis ( J:214828 )

• in vitro assays show a migratory defect in neutrophils in response to the chemoattractant N-formyl-methionine-leucine-phenylalanine (fMLP)

hematopoietic system

abnormal neutrophil morphology ( J:214828 )

• neutrophils show reduced numbers of primary and secondary granules

• neutrophils show alterations in the glycosylation of proteins involved in cell adhesion and cytotoxicity

• however, mice exhibit normal numbers of neutrophils in the bone marrow, secondary lymphoid organs and blood

abnormal neutrophil physiology ( J:214828 )

• neutrophils, but not macrophages and dendritic cells, exhibit impaired killing of Candida albicans

• neutrophils show decreased ability to release myeloperoxidase when challenged in vitro with Candida albicans

impaired neutrophil chemotaxis ( J:214828 )

• in vitro assays show a migratory defect in neutrophils in response to the chemoattractant N-formyl-methionine-leucine-phenylalanine (fMLP)

impaired neutrophil recruitment ( J:214828 )

• recruitment of neutrophils to the peritoneal cavity following Candida albicans injection into the peritoneal cavity is reduced and mice show decreased recruitment of neutrophils to the kidney and lung 24 hours following systemic infection with Candida albicans, indicating that neutrophils cannot effectively migrate to the site of infection