Phenotypes associated with this allele
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh mutation
(6 available);
any
Gt(ROSA)26Sor mutation
(942 available)
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normal phenotype
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• mice are viable and fertile
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gja10tm1.1(icre)Aah mutation
(0 available);
any
Gja10 mutation
(22 available)
Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh mutation
(6 available);
any
Gt(ROSA)26Sor mutation
(942 available)
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vision/eye
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• following infection with adeno-associated viruses that express a single-guide RNA targeting Amigo1 and a fluorescent reporter (AAV2/1-U6-sgAmigo1-CAG-tdTomato), the size of axons of infected horizontal cells is reduced and some branches stray into the outer nuclear layers
• however, the number of dendritic and axonal synapses and the size of horizontal cell dendrites is normal
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nervous system
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• following infection with adeno-associated viruses that express a single-guide RNA targeting Amigo1 and a fluorescent reporter (AAV2/1-U6-sgAmigo1-CAG-tdTomato), the size of axons of infected horizontal cells is reduced and some branches stray into the outer nuclear layers
• however, the number of dendritic and axonal synapses and the size of horizontal cell dendrites is normal
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1(CAG-cas9*,-EGFP)Fezh mutation
(6 available);
any
Gt(ROSA)26Sor mutation
(942 available)
Tmem258tm1.1(KOMP)Vlcg mutation
(0 available);
any
Tmem258 mutation
(13 available)
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cellular
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• after introduction of single guide RNAs (sgRNAs) targeting exonic Tmem258 sequences (Tmem258 CRISPR) by transduction with lentiviral particles co-encoding Cre recombinase, spheroid cultures derived from colonic crypts exhibit fewer viable Cas9-GFP-positive cells than colonic organoid cultures that have received control sgRNAs targeting noncoding intronic Tmem258 regions
• morphologically, Tmem258 CRISPR organoids contain scattered Cas9-GFP-positive cells within small spheroids that encapsulate numerous dead cells within the lumen, whereas control CRISPR organoids appear healthier and larger with contiguous Cas9-GFP-positive cells suggestive of active proliferation
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• after simultaneous delivery of Cre recombinase and sgRNA targeting of Tmem258 sequences by lentiviral transduction, Tmem258 CRISPR organoid cultures contain more in-frame mutations in Cas9-GFP-positive cells than control CRISPR organoids, suggesting that out-of-frame alleles are negatively selected, consistent with increased ER stress
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Analysis Tools